Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse.
Neurochemical characterisation of lamina II inhibitory interneurons that express GFP in the PrP-GFP mouse.
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DOI：
10.1186/1744-8069-9-56
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发表时间：
2013-10-31
期刊：
影响因子：
3.3
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Iwagaki N;Garzillo F;Polgár E;Riddell JS;Todd AJ
脊髓背角浅层的抑制性中间神经元在调节感觉传递中起重要作用，并且这些作用被认为是由不同的功能群体来执行的。我们在大鼠的抑制性中间神经元中鉴定出了4种不重叠的类别，它们是根据甘丙肽、神经肽Y、神经元型一氧化氮合酶（nNOS）和小白蛋白的存在来定义的。生长抑素受体sst2A在该区域约50%的抑制性中间神经元中表达，并且尤其与表达nNOS和甘丙肽的细胞相关。本研究的主要目的是测试一个基因定义的抑制性中间神经元群体，即PrP - GFP小鼠中表达绿色荧光蛋白（GFP）的那些神经元，是否属于在大鼠中鉴定出的一种或多种神经化学类别。
小鼠中sst2A的表达及其与其他神经化学标记物的关系与大鼠相似，只是有相当数量的细胞共同表达nNOS和甘丙肽。PrP - GFP细胞完全包含在具有sst2A受体的抑制性中间神经元集合内，并且几乎所有细胞都表达nNOS和/或甘丙肽。GFP存在于脊髓背角浅层约3 - 4%的神经元中，相当于该区域约16%的抑制性中间神经元。与它们的sst2A免疫反应性一致，所有的GFP细胞都被生长抑素超极化，并且这种情况可通过给予选择性sst2受体拮抗剂或G蛋白偶联的内向整流钾离子通道阻滞剂来阻止。
这些发现支持这样一种观点，即神经化学为脊髓背角浅层抑制性中间神经元的分类提供了一种有价值的方法。结合先前的证据，即PrP - GFP细胞就其生理特性而言形成了一个相对同质的群体，它们表明这些神经元在脊髓背角处理感觉信息方面具有特定的作用。
Inhibitory interneurons in the superficial dorsal horn play important roles in modulating sensory transmission, and these roles are thought to be performed by distinct functional populations. We have identified 4 non-overlapping classes among the inhibitory interneurons in the rat, defined by the presence of galanin, neuropeptide Y, neuronal nitric oxide synthase (nNOS) and parvalbumin. The somatostatin receptor sst2A is expressed by ~50% of the inhibitory interneurons in this region, and is particularly associated with nNOS- and galanin-expressing cells. The main aim of the present study was to test whether a genetically-defined population of inhibitory interneurons, those expressing green fluorescent protein (GFP) in the PrP-GFP mouse, belonged to one or more of the neurochemical classes identified in the rat. The expression of sst2A and its relation to other neurochemical markers in the mouse was similar to that in the rat, except that a significant number of cells co-expressed nNOS and galanin. The PrP-GFP cells were entirely contained within the set of inhibitory interneurons that possessed sst2A receptors, and virtually all expressed nNOS and/or galanin. GFP was present in ~3-4% of neurons in the superficial dorsal horn, corresponding to ~16% of the inhibitory interneurons in this region. Consistent with their sst2A-immunoreactivity, all of the GFP cells were hyperpolarised by somatostatin, and this was prevented by administration of a selective sst2 receptor antagonist or a blocker of G-protein-coupled inwardly rectifying K+ channels. These findings support the view that neurochemistry provides a valuable way of classifying inhibitory interneurons in the superficial laminae. Together with previous evidence that the PrP-GFP cells form a relatively homogeneous population in terms of their physiological properties, they suggest that these neurons have specific roles in processing sensory information in the dorsal horn.
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