A Palm Germ-Radar (PaGeR) for rapid and simple COVID-19 detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
A Palm Germ-Radar (PaGeR) for rapid and simple COVID-19 detection by reverse transcription loop-mediated isothermal amplification (RT-LAMP).
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棕榈胚芽雷达 (PaGeR) 通过逆转录环介导的等温扩增 (RT-LAMP) 快速、简单地检测 COVID-19
DOI：
10.1016/j.bios.2021.113925
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发表时间：
2022-03-15
影响因子：
12.6
通讯作者：
Ma B
中科院分区：
文献类型：
--
作者：
Ge A;Liu F;Teng X;Cui C;Wu F;Liu W;Liu Y;Chen X;Xu J;Ma B
当前，由SARS-CoV-2引发的新冠肺炎疫情正在肆虐，严重威胁着人们的生命安全。建立快速和准确的病原体检测技术不仅对本次疫情至关重要，而且还提醒我们必须时刻为未来可能的疫情做好准备。因此，我们开发了一种Palm Germ-Radar （PaGeR）装置，用于RT-LAMP从提取的患者样本RNA中快速简便地检测COVID-19。COVID-19快速检测的整个过程是基于4个简单步骤：失活、提取、扩增和检测。SARS-CoV-2低至1拷贝/μL，裸眼可选择性检测。该仪器可采用比色法、荧光法和侧量尺读出法三种检测方法。利用PaGeR，我们成功地在拭子标本分离的临床RNA样本中检测到SARS-CoV-2。结果显示，在17名新冠肺炎患者中，有15人被诊断为阳性，而55名正常样本全部被诊断为阴性。因此，所研制的PaGeR检测仪可以实现COVID-19的检测，结果易于可视化，为社区和家庭的快速检测提供了一种很有前景的仪器。
The current COVID-19 pandemic caused by SARS-CoV-2 is raging, seriously threatening people's lives. The establishment of rapid and accurate pathogen detection technology is not only critical in this epidemic, but also a reminder that we must always be prepared for possible future outbreaks. Therefore, we developed a Palm Germ-Radar (PaGeR) device for rapid and simple detection of COVID-19 from extracted patient sample RNA by RT-LAMP. The whole procedure of rapid COVID-19 detection is based on 4 simple steps: inactivation, extraction, amplification, and detection. SARS-CoV-2 down to 1 copy/μL could be detected selectively with naked-eye. Three detection methods (colorimetric, fluorometric and lateral dipstick readout) could be performed in PaGeR instrument. By employing the PaGeR, we successfully detected SARS-CoV-2 in clinical RNA samples isolated from swab specimens. The results showed that 15 out of 17 COVID-19 patients were diagnosed as positive while all 55 normal samples were diagnosed as negative. Therefore, the developed PaGeR instrument can realize the detection of COVID-19 with easily visualized results, providing a promising instrument for rapid detection in the community as well as at home.
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开发和验证快速、单步逆转录酶环介导的等温扩增 (RT-LAMP) 系统，有望用于可靠和高通量的 COVID-19 筛查
影响因子：
5.7
作者：
Jiang, Ming-hua;Pan, Wei-hua;Li, X. J.
通讯作者：
Li, X. J.
影响因子：
4.3
作者：
Barreda-García S;Miranda-Castro R;de-Los-Santos-Álvarez N;Miranda-Ordieres AJ;Lobo-Castañón MJ
通讯作者：
Lobo-Castañón MJ
影响因子：
5.7
作者：
Huang, Wei E.;Lim, Boon;Cui, Zhanfeng
通讯作者：
Cui, Zhanfeng
影响因子：
8.9
作者：
Yao, Yuhan;Chen, Xi;Sui, Guodong
通讯作者：
Sui, Guodong
通过逆转录环介导的等温扩增测定快速直观地检测 2019 新型冠状病毒 (SARS-CoV-2)
影响因子：
14.2
作者：
Yan, C.;Cui, J.;Yuan, J.
通讯作者：
Yuan, J.