Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection.
Helicase-dependent isothermal amplification: a novel tool in the development of molecular-based analytical systems for rapid pathogen detection.
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DOI：
10.1007/s00216-017-0620-3
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发表时间：
2018-01
影响因子：
4.3
通讯作者：
Lobo-Castañón MJ
中科院分区：
文献类型：
--
作者：
Barreda-García S;Miranda-Castro R;de-Los-Santos-Álvarez N;Miranda-Ordieres AJ;Lobo-Castañón MJ
高灵敏度的核酸检测对于改善病原体的检测至关重要，病原体对全球公共卫生构成重大威胁。目前可用的主要基于PCR的分子测定在需要点控制或资源有限的环境中具有有限的效用。因此，人们对开发用于早期检测这些有害微生物的具有成本效益的、鲁棒的和便携式的平台有强烈的兴趣。自2004年描述以来，等温解旋酶依赖性扩增（HDA）已成功应用于开发基于分子的新技术，用于快速，灵敏和选择性检测病毒和细菌。在这篇综述中，我们强调了使用这种简单的核酸扩增方法的相关分析系统，该方法在恒定温度下进行，并且易于与微流体技术兼容。描述了用于监测HDA扩增产物的不同策略。此外，我们提出了整合样品制备，HDA扩增和检测的技术进步。未来的观点和挑战，需要使用点不仅用于临床诊断，而且在食品安全检测和环境监测进行了讨论。利用等温解旋酶依赖性扩增（HDA）扩展用于检测病原体特异性DNA序列的分析工具箱
Highly sensitive testing of nucleic acids is essential to improve the detection of pathogens, which pose a major threat for public health worldwide. Currently available molecular assays, mainly based on PCR, have a limited utility in point-of-need control or resource-limited settings. Consequently, there is a strong interest in developing cost-effective, robust, and portable platforms for early detection of these harmful microorganisms. Since its description in 2004, isothermal helicase-dependent amplification (HDA) has been successfully applied in the development of novel molecular-based technologies for rapid, sensitive, and selective detection of viruses and bacteria. In this review, we highlight relevant analytical systems using this simple nucleic acid amplification methodology that takes place at a constant temperature and that is readily compatible with microfluidic technologies. Different strategies for monitoring HDA amplification products are described. In addition, we present technological advances for integrating sample preparation, HDA amplification, and detection. Future perspectives and challenges toward point-of-need use not only for clinical diagnosis but also in food safety testing and environmental monitoring are also discussed. Expanding the analytical toolbox for the detection of DNA sequences specific of pathogens with isothermal helicase dependent amplification (HDA)
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DOI：
10.1002/anie.201001977
发表时间：
2010-07-26
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
--
作者：
Hoshika S;Chen F;Leal NA;Benner SA
通讯作者：
Benner SA
影响因子：
1.5
作者：
Artiushin, Sergey;Tong, Yanhong;Kong, Huimin
通讯作者：
Kong, Huimin
影响因子：
9.4
作者：
Faron, Matthew L.;Ledeboer, Nathan A.;Buchan, Blake W.
通讯作者：
Buchan, Blake W.
DOI：
10.3390/s150923418
发表时间：
2015-09-16
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Craw P;Mackay RE;Naveenathayalan A;Hudson C;Branavan M;Sadiq ST;Balachandran W
通讯作者：
Balachandran W
DOI：
10.1016/j.diagmicrobio.2011.08.021
发表时间：
2011-12-01
影响因子：
2.9
作者：
Doseeva, Victoria;Forbes, Thomas;Nazarenko, Irina
通讯作者：
Nazarenko, Irina