Characterization of physiological response and identification of associated genes under heat stress in rice seedlings.
Characterization of physiological response and identification of associated genes under heat stress in rice seedlings.
复制标题
水稻幼苗热胁迫下生理反应的表征及相关基因的鉴定。
DOI：
10.1016/j.plaphy.2012.08.011
复制
发表时间：
2012-12
影响因子：
6.5
通讯作者：
薛大伟
中科院分区：
文献类型：
--
作者：
薛大伟
温室气体排放导致的全球变暖使粮食作物更容易受到热胁迫的影响。了解作物的热胁迫相关机制和分类热胁迫相关基因可以增加我们在耐热分子生物学方面的知识，并推动分子设计育种的发展，这可以帮助水稻科普不利的温度。在这项研究中，我们进行了高温胁迫后的水稻植株的生理分析。结果表明，丙二醛含量和超氧化物歧化酶（SOD）活性显著增加。利用改良的SSH筛选长cDNA片段的方法DNSH，成功分离到11个已知功能注释的水稻热相关基因。对公开数据库中的基因芯片数据进行重新分析后发现，这些基因在热胁迫、干旱、寒冷和盐胁迫后均表现出不同的表达模式。实时定量逆转录PCR也进行了验证这些基因的表达后，热应激。除CBL相互作用蛋白激酶contig 77外，其余10个基因的表达均发生显著变化。关于同一基因家族的成员已经发表了几篇报道。
Global warming, which is caused by greenhouse gas emissions, makes food crops more vulnerable to heat stress. Understanding the heat stress-related mechanisms in crops and classifying heat stress-related genes can increase our knowledge in heat-resistant molecular biology and propel developments in molecular design breeding, which can help rice cope with unfavorable temperatures. In this study, we carried out a physiological analysis of rice plants after heat stress. The results show a dramatic increase in malondialdehyde contents and SOD activities. We successfully isolated 11 heat-related rice genes with known function annotation through DNSH, which is an improved SSH method for screening long cDNA fragments. The reanalysis of microarray data from public database revealed that all these genes displayed various expression patterns after heat stress, drought, cold and salt. Quantitative real-time reverse transcription PCR was also performed to validate the expression of these genes after heat stress. The expressions in 10 genes were all significantly changed except for contig 77, which is a CBL-interacting protein kinase. Several reports have been published about the members of the same gene family.
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影响因子：
14.9
作者：
Zhulidov, PA;Bogdanova, EA;Shagin, DA
通讯作者：
Shagin, DA
影响因子：
--
作者：
Cao Liyong
通讯作者：
Cao Liyong
影响因子：
4.3
作者：
Yan Yin;Shumei Li;Weiqin Liao;Qingtao Lu;Xiaogang Wen;Congming Lu
通讯作者：
Yan Yin;Shumei Li;Weiqin Liao;Qingtao Lu;Xiaogang Wen;Congming Lu
DOI：
--
发表时间：
2006
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Zhigang Zhao;Ling Jiang;Yinghui Xiao;Wenwei Zhang;H. Zhai;J. Wan
通讯作者：
Zhigang Zhao;Ling Jiang;Yinghui Xiao;Wenwei Zhang;H. Zhai;J. Wan
影响因子：
4.4
作者：
Liu Q;Wang H;Zhang Z;Wu J;Feng Y;Zhu Z
通讯作者：
Zhu Z