Effects of ecologically relevant concentrations of cadmium on locomotor activity and microbiota in zebrafish.
Effects of ecologically relevant concentrations of cadmium on locomotor activity and microbiota in zebrafish.
复制标题
生态相关镉浓度对斑马鱼运动活动和微生物群的影响。
DOI：
10.1016/j.chemosphere.2020.127220
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发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Meng X
中科院分区：
文献类型：
--
作者：
Xia Y;Zhu J;Xu Y;Zhang H;Zou F;Meng X
镉(Cd)广泛存在于水环境中，对人类和生态系统的影响是一个重要的公共健康问题。然而，它对斑马鱼微生物区系的影响仍然知之甚少。在本研究中，通过7天的水中暴露，评价了生态相关浓度Cd(0，1.2 5，2.5和5μg/L)的潜在发育神经毒性和微生物区系失调。数据显示，暴露于5μg/L的Cd可显著降低存活率，并损害小鼠的运动能力。镉的吸收随暴露浓度的增加和暴露时间的延长而增加。高通量测序分析表明，经Cd处理的斑马鱼微生物区系的丰富度和多样性发生了显着变化。在门水平上，暴露于5μg/L Cd后，变形杆菌的丰度增加，而细菌的丰度则显著降低。在属水平上，与对照组相比，镉处理组与对照组相比，参与神经退行性疾病调控的几种细菌(假单胞菌、反刍菌科、布鲁氏菌、拟杆菌、乳杆菌)的丰度发生了显著变化。此外，染镉组大鼠5-羟色胺信号转导和代谢相关基因bdnf的表达谱也发生了改变。综上所述，这些数据表明，Cd可能通过诱导微生物区系的变化而损害斑马鱼的健康，微生物区系可能成为保护Cd毒害的潜在靶标。
Cadmium (Cd) is widely spread in the aquatic environment, and its impact on humans and the ecosystem is an important issue in public health. However, its effects on zebrafish microbiota are still poorly understood. In this study, the potential developmental neurotoxicity and microbiota dysbiosis of ecologically relevant concentrations of Cd (0, 1.25, 2.5 and 5 μg/L) was evaluated by waterborne exposure for 7 days. The data showed that exposure to 5 μg/L of Cd significantly decreased survival rates and impaired locomotor activities. Uptake of Cd was enhanced with the increase of the concentration and duration of exposure. High-throughput sequencing analysis revealed a significant change in the richness and diversity of the microbiota of Cd-treated zebrafish. At the phylum level, the abundance ofProteobacteriaincreased, while thatFirmicuteswas significantly decreased after exposure to 5 μg/L Cd. At the genus level, there were significant changes in the abundances of several bacteria involved in the regulation of neurodegenerative diseases (Pseudomonas, Ruminococcaceae,Blautia,Bacteroides,Lactobacillus,Lachnospiraceae, andPhascolarctobacterium) in the Cd-treatment groups, as compared to the control group. In addition, the mRNA expression profiles ofbdnfand genes involved in serotonin signaling and metabolism were changed in the Cd exposure groups. Together, these data suggest that Cd could be harmful to zebrafish health by inducing the microbiota changes, and the microbiota could serve as a potential target to protect against the adverse effects of Cd toxicity.
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影响因子：
6.8
作者：
L. Pereira;J. Ribas;T. Vicari;S. B. Silva;J. Stival;A. Baldan;F. X. Valdez Domingos;M. Grassi;M. Cestari;H. C. Silva de Assis
通讯作者：
L. Pereira;J. Ribas;T. Vicari;S. B. Silva;J. Stival;A. Baldan;F. X. Valdez Domingos;M. Grassi;M. Cestari;H. C. Silva de Assis
影响因子：
11.9
作者：
Rajakovich LJ;Balskus EP
通讯作者：
Balskus EP
影响因子：
2.7
作者：
Melissa N. Conley;C. Wong;Kyle M Duyck;N. Hord;E. Ho;T. Sharpton
通讯作者：
Melissa N. Conley;C. Wong;Kyle M Duyck;N. Hord;E. Ho;T. Sharpton
影响因子：
15.1
作者：
Robertson, Ruairi C.;Oriach, Clara Seira;Stanton, Catherine
通讯作者：
Stanton, Catherine
影响因子：
8.8
作者：
He, Xiwei;Qi, Zhaodong;Zhang, Xu-Xiang
通讯作者：
Zhang, Xu-Xiang