Authentication of the anti-tumor herb Baihuasheshecao with bioactive marker compounds and molecular sequences.
Authentication of the anti-tumor herb Baihuasheshecao with bioactive marker compounds and molecular sequences.
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DOI：
10.1016/j.foodchem.2009.09.013
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发表时间：
2010-04-01
期刊：
影响因子：
8.8
通讯作者：
But, Paul Pui-Hay
中科院分区：
文献类型：
--
作者：
Li, Ming;Jiang, Ren-Wang;Hon, Po-Ming;Cheng, Ling;Li, Ling-Lin;Zhou, Jin-Rong;Shaw, Pang-Chui;But, Paul Pui-Hay
关键词：
白花蛇舌草（白花蛇舌草）是一种用于治疗癌症的中药，经常被一种相关物种伞房花蛇舌草掺杂。应用全内转录间隔区DNA序列测定方法鉴别H. diffusa来自H. corymbosa和其他密切相关的物种。分子数据显示，七份白花蛇舌草药材样品中有四份是掺假品。采用薄层色谱法和高效液相色谱法对H. diffusa和H.伞房花序。在H. diffusa：6-O-（E）-p-香豆酰基斯堪的纳维亚苷甲酯（化合物1）和10（S）-羟基脱镁叶绿素a（化合物2）。两种化合物对PC 3人雄激素非依赖性前列腺癌细胞均显示出中等的抗增殖作用，而化合物2对LNCaP人雄激素敏感性前列腺癌细胞也显示出强的抗增殖作用。因此，这些生物活性标记化合物可用于白花蛇舌草的真伪鉴别和质量评价。
Baihuasheshecao (Hedyotis diffusa), a Chinese herb for cancer treatment, is frequently adulterated by a related species Hedyotis corymbosa. DNA sequencing of the complete internal transcribed spacer region was applied to differentiate H. diffusa from H. corymbosa and other closely related species. The molecular data showed that four out of seven herb samples of Baihuasheshecao were adulterants. Chemical analyses by TLC and HPLC were used to authenticate H. diffusa and H. corymbosa. Two marker compounds were identified exclusively in H. diffusa: 6-O-(E)-p-coumaroyl scandoside methyl ester (compound 1) and 10(S)-hydroxypheophytin a (compound 2). Both compounds showed moderate anti-proliferation effect on PC3 human androgen-independent prostate cancer cells, while compound 2 also showed strong anti-proliferation effect on LNCaP human androgen-sensitive prostate cancer cells. Accordingly, these bioactive marker compounds could be applied to verify the authenticity and assess the quality of Baihuasheshecao.
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影响因子：
3.8
作者：
Jiang, Ren-Wang;Hay, Mark E.;Fairchild, Craig R.;Prudhomme, Jacques;Le Roch, Karine;Aalbersberg, William;Kubanek, Julia
通讯作者：
Kubanek, Julia
影响因子：
5.1
作者：
Jiang, Ren-Wang;Zhou, Jin-Rong;But, Paul Pui-Hay
通讯作者：
But, Paul Pui-Hay
影响因子：
1.7
作者：
Liang, Zhi-Tao;Jiang, Zhi-Hong;Zhao, Zhong-Zhen
通讯作者：
Zhao, Zhong-Zhen
影响因子：
5.1
作者：
DAI, R;SHOEMAKER, R;SONG, PS
通讯作者：
SONG, PS
影响因子：
5.7
作者：
Lin, CC;Ng, LT;Yang, JJ
通讯作者：
Yang, JJ