Ω3 Supplementation and intermittent hypobaric hypoxia induce cardioprotection enhancing antioxidant mechanisms in adult rats.
Ω3 Supplementation and intermittent hypobaric hypoxia induce cardioprotection enhancing antioxidant mechanisms in adult rats.
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DOI：
10.3390/md13020838
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发表时间：
2015-02-04
期刊：
影响因子：
5.4
通讯作者：
Castillo RL
中科院分区：
文献类型：
--
作者：
Herrera EA;Farías JG;González-Candia A;Short SE;Carrasco-Pozo C;Castillo RL
间歇性低压缺氧（IH）与氧化应激有关，损害心脏功能。然而，早期IH也激活心脏保护机制。Omega 3脂肪酸（Ω3）通过减少梗死面积和增强抗氧化防御来诱导心脏保护作用。这项工作的目的是确定IH和β-内酰胺酶3对心脏功能、氧化平衡和炎症状态的联合作用。将28只大鼠随机分为4组：常压常氧（N）组; N + NH3（0.3 g·kg-1·day-1）组; IH组;和IH + NH3组。在32天内，在低压舱中通过4个间歇期的缺氧（4天）-常氧（4天）诱导IH。在暴露结束时，将心脏固定在Langendorff系统中，并进行30分钟的缺血，随后进行120分钟的再灌注。此外，我们还通过丙二醛和硝基酪氨酸定量测定了HIF-1α和ATP水平以及氧化应激。此外，抗氧化酶超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶的表达进行了测定。在心脏中评估NF-κ B和髓过氧化物酶水平。相对于N个心脏，IH改善了左心室功能（左心室发展压力：N; 21.8 ± 3.4 vs. IH; 42.8 ± 7.1 mmHg; p < 0.05）;降低了氧化应激（丙二醛：N; 14.4 ± 1.8 vs. IH; 7.3 ± 2.1 μmol/mg蛋白; p < 0.05）;和增加的抗氧化酶表达。补充抗氧化剂3诱导与IH组相似的反应。我们的研究结果表明，IH和β-淀粉样蛋白3以独立的方式通过抗氧化和抗炎机制诱导功能改善，建立心脏保护。
Intermittent hypobaric hypoxia (IH) is linked with oxidative stress, impairing cardiac function. However, early IH also activate cardio-protective mechanisms. Omega 3 fatty acids (Ω3) induce cardioprotection by reducing infarct size and reinforcing antioxidant defenses. The aim of this work was to determine the combined effects of IH and Ω3 on cardiac function; oxidative balance and inflammatory state. Twenty-eight rats were randomly divided into four groups: normobaric normoxia (N); N + Ω3 (0.3 g·kg−1·day−1); IH; and IH + Ω3. IH was induced by 4 intercalate periods of hypoxia (4 days)—normoxia (4 days) in a hypobaric chamber during 32 days. At the end of the exposure, hearts were mounted in a Langendorff system and subjected to 30 min of ischemia followed by 120 min of reperfusion. In addition, we determined HIF-1α and ATP levels, as well as oxidative stress by malondialdehyde and nitrotyrosine quantification. Further, the expression of the antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase was determined. NF-kappaB and myeloperoxidase levels were assessed in the hearts. Relative to N hearts, IH improved left ventricular function (Left ventricular developed pressure: N; 21.8 ± 3.4 vs. IH; 42.8 ± 7.1 mmHg; p < 0.05); reduced oxidative stress (Malondialdehyde: N; 14.4 ± 1.8 vs. IH; 7.3 ± 2.1 μmol/mg prot.; p < 0.05); and increased antioxidant enzymes expression. Supplementation with Ω3 induces similar responses as IH group. Our findings suggest that both, IH and Ω3 in an independent manner, induce functional improvement by antioxidant and anti-inflammatory mechanisms, establishing cardio-protection.
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影响因子：
11
作者：
Chiu, LL;Chou, SW;Kuo, CH
通讯作者：
Kuo, CH
影响因子：
5.5
作者：
Chen, CF;Tsai, SY;Wu, MS
通讯作者：
Wu, MS
缺氧影响大鼠骨骼肌中线粒体蛋白的表达。
影响因子：
3.3
作者：
Chen, Jian;Gao, Yuqi;Gao, Wenxiang
通讯作者：
Gao, Wenxiang
DOI：
10.1016/j.jtcvs.2010.04.030
发表时间：
2011-03-01
影响因子：
6
作者：
Byrne, John;McGuinness, Jonathan;Redmond, Mark J.
通讯作者：
Redmond, Mark J.
影响因子：
5.5
作者：
Bailey, Damian M.;Dehnert, Christoph;Berger, Marc M.
通讯作者：
Berger, Marc M.