In vitro shoot organogenesis and plant regeneration in Tibouchina aspera Aubl.
In vitro shoot organogenesis and plant regeneration in Tibouchina aspera Aubl.
复制标题
Tibouchina aspera Aubl 的体外芽器官发生和植物再生。
DOI：
10.1007/s11627-015-9691-z
复制
发表时间：
2015-05
期刊：
影响因子：
--
通讯作者：
Guohua Ma
中科院分区：
文献类型：
--
作者：
Xinhua Zhang;Seping Dai;Jaime A. Teixeira da Silva;Guohua Ma
麻豆科是植物界第七大科，约170属4600种，广泛分布在世界热带和亚热带地区（the plant List 2015a）。其中，Tibouchina属由约400种组成，许多原产于巴西，但也分布在墨西哥和西印度群岛到阿根廷北部（Renner 1993; the Plant List 2015b）。许多种类的藏红花被用作观赏和药用植物。前一类植物用作床上植物或盆栽植物，后一类植物的类黄酮具有积极的健康相关属性（Ciampolini et al. 1995; Yoshida et al. 1999a,b; Dixon et al. 2005）和抗真菌特性（Kuster et al. 2009）。Tibouchina的花通常是紫色的，富含花青素，作为人类饮食的一部分有着悠久的历史（Bobbio et al. 1985; Janna et al. 2006; Chan et al. 2008）。Tibouchina aspera Aubl。，原产于热带美洲（Funk et al. 2007），是一种常绿灌木，具有异域情调，毛茸茸的，有光泽的灰色叶子，从初夏到秋天都有顶生花朵。虽然在几年前被引进到中国南方，但它在那种气候下不产籽。为了更好地开发利用这一观赏药用植物，有必要对其进行育种。建立有效的离体微繁和植株再生方案将使这一目标得以实现。本研究首次采用未成熟叶片外植体诱导萌发新梢。建立了高效的离体繁殖和植株再生体系，实现了驯化植株开花。此外，还研究了不同植物生长调节剂（pgr）在芽诱导培养基中的作用。
TheMelastomataceae is the seventh largest family in the plant kingdom and contains approximately 170 genera and 4600 species widely distributed in tropical and subtropical regions of the world (The Plant List 2015a). Among these, the genus Tibouchina is made up of about 400 species, many native to Brazil but also ranging from Mexico and the West Indies to northern Argentina (Renner 1993; The Plant List 2015b). Many species of Tibouchina are used as ornamental and medicinal plants. Those in the former category serve as bedding or potted plants, while members of the latter category have flavonoids associated with positive health-related attributes (Ciampolini et al. 1995; Yoshida et al. 1999a,b; Dixon et al. 2005) and antifungal properties (Kuster et al. 2009). The flowers of Tibouchina are usually purple and rich in anthocyanins, with a long history as part of the human diet (Bobbio et al. 1985; Janna et al. 2006; Chan et al. 2008). Tibouchina aspera Aubl., which originated in tropical America (Funk et al. 2007), is an evergreen shrub with exotic, fuzzy, lustrous grayish foliage, and acrogenous flowers that appear from early summer to autumn. Although T. asperawas introduced to South China several years ago, it does not produce seed in that climate. In a bid to better exploit and utilize this ornamental and medicinal plant, it is essential to propagate the species. The establishment of an efficient in vitro micropropagation and plant regeneration protocol would allow this objective to be achieved. In this study, shoots were induced in T. aspera from immature leaf explants for the first time. An efficient in vitro propagation and plant regeneration system was established, and flowering of acclimatized plants was achieved. The effects of light versus darkness and of different plant growth regulators (PGRs) in shoot induction media were also investigated.
登录
查看更多内容
影响因子：
3.7
作者：
Wenling Zhou;Rufang Tan;Chuanjun Xu;Yanyan Lai;Dongyin Chen;Ling Li
通讯作者：
Wenling Zhou;Rufang Tan;Chuanjun Xu;Yanyan Lai;Dongyin Chen;Ling Li
DOI：
--
发表时间：
2007
期刊：
--
影响因子：
--
作者：
G. Aymard;P. Berry;R. Cowan;N. Alvarado;A. Salinas;P. R. Fantz;R. Maxwell;K. M. Redden;V. E. Rudd;M. Sousa;D. Wind
通讯作者：
G. Aymard;P. Berry;R. Cowan;N. Alvarado;A. Salinas;P. R. Fantz;R. Maxwell;K. M. Redden;V. E. Rudd;M. Sousa;D. Wind
影响因子：
5.2
作者：
Gallego, SM;Benavides, MP;Tomaro, ML
通讯作者：
Tomaro, ML
DOI：
--
发表时间：
2009
期刊：
--
影响因子：
--
作者：
G. Ma;Yong Li;G. Jiao;Xiao-ping Fu;Yourun Lin
通讯作者：
G. Ma;Yong Li;G. Jiao;Xiao-ping Fu;Yourun Lin
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F