Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio).
Compensatory regulation of Na+ absorption by Na+/H+ exchanger and Na+-Cl- cotransporter in zebrafish (Danio rerio).
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DOI：
10.1186/1742-9994-10-46
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发表时间：
2013-08-07
影响因子：
2.8
通讯作者：
Hwang PP
中科院分区：
文献类型：
--
作者：
Chang WJ;Wang YF;Hu HJ;Wang JH;Lee TH;Hwang PP
在哺乳动物中，内部Na+稳态是通过多种具有相互补偿功能的Na+转运蛋白的Na+重吸收来维持的，这些蛋白在肾单位的不同部分表达。在斑马鱼中，Na+稳态主要通过皮肤/鳃离子细胞实现，即表达Na+/H+交换器（NHE3b）的富含H+-ATP酶（HR）细胞和表达Na+-Cl-协同转运蛋白（NCC）的NCC细胞，它们在功能上与哺乳动物近端和远端曲管细胞同源。 分别。本研究旨在调查 HR 和 NCC 离子细胞的功能是否受到差异性调节以补偿内部 Na+ 稳态的破坏，以及离子细胞的细胞分化是否参与该调节途径。 ncc 的翻译敲除导致 HR 细胞数量增加，从而增加斑马鱼幼虫对 Na+ 的吸收，而 NHE3b 功能丧失则导致 NCC 细胞数量增加，同时 Na+ 吸收恢复。环境酸胁迫抑制HR细胞中nhe3b的表达并降低Na+含量，随后NCC细胞上调并伴随Na+含量的恢复。此外，ncc 的敲低导致酸适应斑马鱼的 Na+ 含量显着降低。这些结果证明HR和NCC细胞在Na+吸收方面表现出功能冗余，类似于哺乳动物肾脏的调节机制，并表明这种功能冗余是斑马鱼在扰乱体液Na+稳态的恶劣环境中生存的关键策略。
In mammals, internal Na+ homeostasis is maintained through Na+ reabsorption via a variety of Na+ transport proteins with mutually compensating functions, which are expressed in different segments of the nephrons. In zebrafish, Na+ homeostasis is achieved mainly through the skin/gill ionocytes, namely Na+/H+ exchanger (NHE3b)-expressing H+-ATPase rich (HR) cells and Na+-Cl- cotransporter (NCC)-expressing NCC cells, which are functionally homologous to mammalian proximal and distal convoluted tubular cells, respectively. The present study aimed to investigate whether or not the functions of HR and NCC ionocytes are differentially regulated to compensate for disruptions of internal Na+ homeostasis and if the cell differentiation of the ionocytes is involved in this regulation pathway. Translational knockdown of ncc caused an increase in HR cell number and a resulting augmentation of Na+ uptake in zebrafish larvae, while NHE3b loss-of-function caused an increase in NCC cell number with a concomitant recovery of Na+ absorption. Environmental acid stress suppressed nhe3b expression in HR cells and decreased Na+ content, which was followed by up-regulation of NCC cells accompanied by recovery of Na+ content. Moreover, knockdown of ncc resulted in a significant decrease of Na+ content in acid-acclimated zebrafish. These results provide evidence that HR and NCC cells exhibit functional redundancy in Na+ absorption, similar to the regulatory mechanisms in mammalian kidney, and suggest this functional redundancy is a critical strategy used by zebrafish to survive in a harsh environment that disturbs body fluid Na+ homeostasis.
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影响因子：
0.9
作者：
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通讯作者：
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