Genetic alterations and growth pattern in biliary duct carcinomas: loss of heterozygosity at chromosome 5q bears a close relation with polypoid growth
Genetic alterations and growth pattern in biliary duct carcinomas: loss of heterozygosity at chromosome 5q bears a close relation with polypoid growth
复制标题
胆管癌的遗传改变和生长模式：5q染色体杂合性缺失与息肉样生长密切相关
作者：
E. Hidaka;A. Yanagisawa;M. Seki;T. Setoguchi;Y. Kato
胆管癌（BDC）是相对罕见的，其诱导的致癌机制知之甚少。有两种生长模式，息肉状和非息肉状浸润型，但很少有关于生长模式和遗传变异之间的关系的信息。因此，进行了比较研究，以澄清在息肉样和非息肉样浸润型BDC之间是否存在遗传变化的差异，包括5 q、9 p、17 p和18 q处的杂合性丢失（洛）和K-ras突变。用微卫星标记进行洛缺失分析，用斑点杂交分析K-ras点突变。息肉样和非息肉样浸润型在5 q、9 p、17 p、18 q的发生率分别为73%和26%、63%和59%、55%和50%、20%和18%，K-ras突变发生率分别为25%和27%。最重要的是，我们发现息肉样生长的5 qLOH频率显著高于非息肉样浸润型（p<0.05），尤其是在肝外胆管癌中（p<0.05）。其他遗传改变（9 p、17 p和18 q处的洛合性缺失和K-ras突变）的发生率在两种肿瘤类型中相似。目前的数据表明，5 qLOH可能有密切的关系，息肉样生长的BDC。
Biliary duct carcinomas (BDCs) are relatively rare and the carcinogenic mechanisms underlying their induction are poorly understood. There are two growth patterns, polypoid and non-polypoid infiltrative type, but little information is available concerning the relation between growth pattern and genetic alterations. A comparative study was therefore conducted to clarify if differences in genetic changes, including loss of heterozygosity (LOH) at 5q, 9p, 17p, and 18q, and K-ras mutations exist between polypoid and non-polypoid infiltrative type BDCs. LOH analysis was performed using microsatellite markers and K-ras point mutations were analysed by dot blot hybridisation. The incidences of changes for polypoid and non-polypoid infiltrative types were 73% and 26% on 5q, 63% and 59% on 9p, 55% and 50% on 17p, and 20% and 18% on 18q, and 25% and 27% for K-ras mutations. Most importantly, we found the frequency of 5qLOH to be significantly higher with polypoid growth than in the non-polypoid infiltrative type (p<0.05), especially in extrahepatic duct carcinomas (p<0.05). The incidences of other genetic alterations (LOH at 9p, 17p, and 18q, and K-ras mutations) showed similar rates with both tumour types. The present data suggest that 5qLOH may have a close relation with polypoid growth in BDCs.
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影响因子：
11.2
作者：
I. Wistuba;K. Sugio;J. Hung;Y. Kishimoto;A. Virmani;I. Roá;J. Albores–Saavedra;A. Gazdar
通讯作者：
I. Wistuba;K. Sugio;J. Hung;Y. Kishimoto;A. Virmani;I. Roá;J. Albores–Saavedra;A. Gazdar
影响因子：
56.9
作者：
NISHISHO, I;NAKAMURA, Y;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
56.9
作者：
SU, LK;VOGELSTEIN, B;KINZLER, KW
通讯作者：
KINZLER, KW
影响因子：
158.5
作者：
VOGELSTEIN, B;FEARON, ER;BOS, JL
通讯作者：
BOS, JL
影响因子：
56.9
作者：
KAMB, A;GRUIS, NA;SKOLNICK, MH
通讯作者：
SKOLNICK, MH