Poly(ethylene glycol) cross-linked hemoglobin with antioxidant enzymes protects pancreatic islets from hypoxic and free radical stress and extends islet functionality.
Poly(ethylene glycol) cross-linked hemoglobin with antioxidant enzymes protects pancreatic islets from hypoxic and free radical stress and extends islet functionality.
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DOI：
10.1002/bit.24501
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发表时间：
2012-09
影响因子：
3.8
通讯作者：
Bae, You Han
中科院分区：
文献类型：
--
作者：
Nadithe, Venkatareddy;Mishra, Deepa;Bae, You Han
本研究的目的是研究在模拟移植条件下，以聚乙二醇为基础的多功能血红蛋白缀合物与抗氧化酶交联的效率，以保护氧载体（血红蛋白）和胰岛素分泌的胰岛免受缺氧和自由基应激的影响。在这项研究中，RINm5F细胞和分离的胰岛被氧化剂（H2O2或黄嘌呤和黄嘌呤氧化酶）激发，并与偶联物（Hb-Hb或Hb-SOD-CAT）在常氧（21%氧气）或缺氧（6%或1%氧气）下孵育。高铁血红蛋白法、DCF-DA法和MTT法分别测定了RINm5F细胞的血红蛋白保护作用、细胞内自由基活性和细胞活力，结果表明含有抗氧化酶的缀合物对细胞的保护作用更好。胰岛的胰岛素分泌和定性共聚焦评价表明，β细胞在暴露于诱导应激时受到含有抗氧化酶的缀合物的保护。我们的研究表明，抗氧化酶在血红蛋白保护中起着重要作用，从而延长了细胞保护。
The objective of this study was to investigate the efficiency of multifunctional PEG-based hemoglobin conjugates crosslinked with antioxidant enzymes for their ability to protect an oxygen carrier (hemoglobin) and insulin secreting islets from the combination of hypoxic and free radical stress under simulated transplantation conditions. In this study, RINm5F cells and isolated pancreatic islets were challenged with oxidants (H2O2 or xanthine and xanthine oxidase) and incubated with conjugates (Hb-Hb or Hb-SOD-CAT) in normoxia (21% oxygen) or hypoxia (6% or 1% oxygen). Hemoglobin protection, intracellular free radical activity and cell viability in RINm5F cells measured by methemoglobin, DCF-DA and MTT assay respectively showed that cells were better protected by conjugates containing antioxidant enzymes. Insulin secretion from islets and qualitative confocal evaluation of viability showed beta cells were protected by conjugates containing antioxidant enzymes when exposed to induced stress. Our study suggested that antioxidant enzymes play a significant role in hemoglobin protection and thus extended cell protection.
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DOI：
10.1016/j.ijbiomac.2010.08.007
发表时间：
2010-12-01
影响因子：
8.2
作者：
Nadithe V;Bae YH
通讯作者：
Bae YH
影响因子：
7.4
作者：
Lenzen, S;Drinkgern, J;Tiedge, M
通讯作者：
Tiedge, M
影响因子：
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作者：
Chae, SY;Kim, SW;Bae, YH
通讯作者：
Bae, YH
影响因子：
7.7
作者：
DIONNE, KE;COLTON, CK;YARMUSH, ML
通讯作者：
YARMUSH, ML
影响因子：
4.8
作者：
Imoto, Hirofumi;Sasaki, Nobuhiro;Iida, Mitsuo
通讯作者：
Iida, Mitsuo