Synthesis and characterization of hemoglobin conjugates with antioxidant enzymes via poly(ethylene glycol) cross-linker (Hb-SOD-CAT) for protection from free radical stress.
Synthesis and characterization of hemoglobin conjugates with antioxidant enzymes via poly(ethylene glycol) cross-linker (Hb-SOD-CAT) for protection from free radical stress.
复制标题
DOI：
10.1016/j.ijbiomac.2010.08.007
复制
发表时间：
2010-12-01
影响因子：
8.2
通讯作者：
Bae YH
中科院分区：
文献类型：
--
作者：
Nadithe V;Bae YH
利用二羧基甲基化聚乙二醇偶联抗氧化酶（SOD和CAT），设计血红蛋白（Hb）以保护血红蛋白免受自由基侵害。本研究采用SDS-PAGE和SEC技术对其偶联过程进行了验证。偶联物的平均分子量估计在1000 kDa左右。偶联物（Hb-SOD-CAT）中SOD和CAT的酶活性分别保持了原来生物活性的70%和90%以上。结果表明，在偶联过程中，抗氧化酶有助于减少高铁血红蛋白（非氧载体）的形成，并在4°C下保存一个月。综上所述，在过氧化氢（0.1和1mM）和黄嘌呤(1mM)/黄嘌呤氧化酶（10和20mUnits/mL）体系中培养时，优化的（1:10 Hb/PEG）交联抗氧化酶偶联物对严重自由基胁迫具有保护作用。
Hemoglobin (Hb) conjugated with the antioxidant enzymes (SOD and CAT), by employing dicarboxymethylated poly(ethylene glycol), was designed for protection of hemoglobin against free radicals. In this study, the conjugation process was confirmed by employing SDS-PAGE and SEC techniques. The average molecular weight of the conjugates was estimated to be around 1000 kDa. The enzymatic activities of the SOD and CAT in the conjugates (Hb-SOD-CAT) after conjugation were found to retain greater than 70% and 90% of the original bioactivity. Results show that antioxidant enzymes helped minimize methemoglobin (non-carrier of oxygen) formation during the conjugation process and also during storage at 4°C over a period of one month. In summary, the optimized (1:10 Hb/PEG) cross-linked conjugates with antioxidant enzymes showed protective properties from severe free radical stresses when incubated with hydrogen peroxide (0.1 and 1mM) and xanthine (1mM)/xanthine oxidase (10 and 20mUnits/mL) system.
登录
查看更多内容
现代的交联策略，用于合成细胞细胞血红蛋白的氧气载体。
影响因子：
2.9
作者：
Harris, David Raphael;Palmer, Andre Francis
通讯作者：
Palmer, Andre Francis
DOI：
10.1002/jbm.820240905
发表时间：
1990-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
HUANGLEE, LLH;CHEUNG, DT;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
--
作者：
Chae, SY;Kim, SW;Bae, YH
通讯作者：
Bae, YH
影响因子：
8.8
作者：
Gotshall, Robert W.;Hamilton, Karyn L.;Irwin, David C.
通讯作者：
Irwin, David C.
DOI：
10.1016/s0006-291x(02)00310-8
发表时间：
2002-05-10
影响因子：
3.1
作者：
Hu, T;Su, ZG
通讯作者：
Su, ZG