Multidimensional mass spectrometry-based shotgun lipidomics.
Multidimensional mass spectrometry-based shotgun lipidomics.
复制标题
基于多维质谱的鸟枪脂质组学。
DOI：
10.1007/978-1-4939-1258-2_13
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Han,Xianlin
中科院分区：
文献类型：
--
作者：
Wang,Miao;Han,Xianlin
基于多维质谱技术的鸟枪脂组学(MDMS-SL)以其高效、灵敏、重复性好、覆盖面广等优点，已成为当前脂肪组学研究的基础分析技术平台。该平台已被广泛用于确定由疾病、基因操作、药物治疗和衰老等引起的脂类、亚类和单个分子物种的含量和/或组成的改变。在这里，我们简要讨论了这项技术的基本原理，并提出了一种常规分析MDMS-SL直接从生物样品的脂类提取物中分析许多脂类和亚类的协议。特别是对MDMS-SL的关键组成部分之一--从各种生物材料中制备脂质样品进行了详细的描述。只要进行适当的样品制备，质谱分析的方法可以很容易地扩展到其他未提及的脂类的分析，并应有助于该领域的研究人员更好地理解和管理细胞脂体的分析技术。
Multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) has become a foundational analytical technology platform among current lipidomics practices due to its high efficiency, sensitivity, and reproducibility, as well as its broad coverage. This platform has been broadly used to determine the altered content and/or composition of lipid classes, subclasses, and individual molecular species induced by diseases, genetic manipulations, drug treatments, and aging, among others. Herein, we briefly discuss the principles underlying this technology and present a protocol for routine analysis of many of the lipid classes and subclasses covered by MDMS-SL directly from lipid extracts of biological samples. In particular, lipid sample preparation from a variety of biological materials, which is one of the key components of MDMS-SL, is described in detail. The protocol for mass spectrometric analysis can readily be expanded for analysis of other lipid classes not mentioned as long as appropriate sample preparation is conducted, and should aid researchers in the field to better understand and manage the technology for analysis of cellular lipidomes.
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影响因子：
2.9
作者：
Jiang, Xuntian;Cheng, Hua;Han, Xianlin
通讯作者：
Han, Xianlin
影响因子：
2.1
作者：
Han, Xianlin
通讯作者：
Han, Xianlin
影响因子：
2
作者：
Jiang, Xuntian;Ory, Daniel S.;Han, Xianlin
通讯作者：
Han, Xianlin
影响因子：
7.4
作者：
Li, Ying L.;Su, Xiong;Gross, Michael L.
通讯作者：
Gross, Michael L.
影响因子：
7.4
作者：
Yang, Kui;Zhao, Zhongdan;Gross, Richard W.;Han, Xianlin
通讯作者：
Han, Xianlin