Isolated chick osteocytes stimulate formation and bone-resorbing activity of osteoclast-like cells
Isolated chick osteocytes stimulate formation and bone-resorbing activity of osteoclast-like cells
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分离的鸡骨细胞刺激破骨细胞样细胞的形成和骨吸收活性
DOI：
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发表时间：
2005
影响因子：
3.3
通讯作者：
Y. Hakeda
中科院分区：
文献类型：
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作者：
Kayo Tanaka;Y. Yamaguchi;Y. Hakeda
骨细胞在骨代谢中有几种可能的作用，但这些细胞的确切功能仍然不清楚。在这项研究中，我们研究了骨细胞对破骨细胞形成的影响，使用分离的骨细胞从16日龄的鸡胚顶骨和小鼠造血母细胞从脾细胞从5-氟尿嘧啶治疗的小鼠。分离的骨细胞的纯度超过95%，其骨细胞表型，如具有典型的星状形状和骨细胞特异性单克隆抗体OB 7.3的免疫反应，非增殖表型，和那些阴性的碱性磷酸酶（ALP）的活性，在3天的培养期间被保留。当造血母细胞与分离的骨细胞共培养时，即使在不存在任何促骨因子的情况下，来自母细胞的抗酒石酸盐酸性磷酸酶（TRAP）阳性多核细胞（MNCs）的数量也会增加。这种刺激作用取决于加入的骨细胞的数量，并且与通过剥离骨膜获得的无非骨细胞细胞的骨碎片一致。分离的骨细胞的条件培养基（CM）对MNCs的形成和骨吸收活性也具有刺激作用，表明骨细胞至少部分地通过产生一些可溶性因子来增加骨细胞的发育。在从mono-Q阴离子交换柱以约0.3 M NaCl洗脱的级分中观察到骨细胞CM中的这种破骨细胞诱导活性，并且这种洗脱曲线与新鲜分离的骨细胞和剥离的骨碎片的细胞裂解物的洗脱曲线相似，表明体内存在这种破骨细胞生成因子。最后，骨细胞CM和mono-Q层析的活性组分也增加了未分级小鼠骨细胞培养物中牙本质切片上的小凹面积。因此，骨细胞可能在骨吸收的生理过程中发挥作用。
Several possible roles have been proposed for osteocytes in bone metabolism, but the precise functions of these cells are still obscure. In this study, we examined the effects of osteocytes on osteoclast formation, using isolated osteocytes from 16-day-old chick embryonic parietal bones and mouse hemopoietic blast cells from spleen cells obtained from 5-fluorouracil-treated mice. Purity of the isolated osteocytes was more than 95%, and their osteocytic phenotypes—such as those having a typical stellate shape and being immunoreactive for osteocyte-specific monoclonal antibody OB 7.3, the nonproliferative phenotype, and those negative for alkaline phosphatase (ALP) activity—were retained during 3 days of culture. When hemopoietic blast cells were cocultured with the isolated osteocytes, the number of osteoclastic tartrate-resistant acid phosphatase (TRAP)-positive multinucleate cells (MNCs) from the blast cells increased even in the absence of any osteotropic factors. This stimulatory effect depended on the number of osteocytes added, and was consistent with that obtained with bone fragments freed of nonosteocytic cells by stripping the periosteum. Conditioned medium (CM) of isolated osteocytes also had stimulatory effects on both formation and bone-resorbing activity of the MNCs, indicating that osteocytes increased osteoclastic development, at least in part, via their production of some soluble factor(s). This osteoclast-inducing activity in the osteocyte CM was observed in the fraction that eluted at around 0.3 M NaCl from a mono-Q anion-exchange column, and this elution profile was similar to that of cell lysates of freshly isolated osteocytes and of stripped bone fragments, indicating the existence of such osteoclastogenesis factor(s) in vivo. Finally, both the osteocyte CM and the active fraction of mono-Q chromatography increased the pit area on dentine slices in the cultures of unfractionated mouse bone cells as well. Thus, osteocytes may play a role in the physiological processes of osteoclastic bone resorption.
在颅盖条件培养基中培养的骨髓中破骨细胞的发育。
影响因子：
2.7
作者：
Oursler,MJ;Osdoby,P
通讯作者：
Osdoby,P
富含粒细胞-巨噬细胞祖细胞的骨髓部分在体外产生破骨细胞。
影响因子：
4.1
作者：
Schneider,GB;Relfson,M
通讯作者：
Relfson,M