A gene-for-gene interaction involving a 'late' effector contributes to quantitative resistance to the stem canker disease in Brassica napus.
A gene-for-gene interaction involving a 'late' effector contributes to quantitative resistance to the stem canker disease in Brassica napus.
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DOI：
10.1111/nph.17292
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发表时间：
2021-08
期刊：
影响因子：
--
通讯作者：
Rouxel T
中科院分区：
文献类型：
--
作者：
Jiquel A;Gervais J;Geistodt-Kiener A;Delourme R;Gay EJ;Ollivier B;Fudal I;Faure S;Balesdent MH;Rouxel T
甘蓝型油菜茎溃烂病的防治主要基于植物遗传抗性：单基因特异性抗性(RLM基因)或数量多基因成虫期抗性。我们的工作假说是，数量抗性部分遵循基因对基因模型，抗性基因在系统定植后期表达“识别”真菌的效应因子。选择了5个茎表达效应基因，并将其置于AvrLm4-7启动子的控制下，用于小型子叶接种试验，筛选了204个油菜品种的基因库。我们鉴定了一个对LmSTEE98敏感的油菜品种‘豫大’。通过CRISPR-Cas9方法使LmSTEE98基因失活，进一步验证了LmSTEE98-RlmSTEE98的相互作用。带有LmSTEE98突变株系的菌株在‘豫大’上比野生型菌株产生了更严重的茎症状。这一单基因抗性被定位在‘达摩_bzh’ב豫大’遗传图谱的0.6 cM区间内。一种典型的基因对基因的相互作用在一定程度上导致了斑点落叶松毛虫(L. maculans)在油菜茎上定殖时的数量抗性。我们的研究为抗病基因的发现、数量抗性机制的阐明和持久抗病的选择提供了新的途径。另见Venault-Fourrey&Rep对这篇文章的评论，231：1301-1303。
The control of stem canker disease of Brassica napus (rapeseed), caused by the fungus Leptosphaeria maculans is based largely on plant genetic resistance: single‐gene specific resistance (Rlm genes) or quantitative, polygenic, adult‐stage resistance. Our working hypothesis was that quantitative resistance partly obeys the gene‐for‐gene model, with resistance genes ‘recognizing’ fungal effectors expressed during late systemic colonization. Five LmSTEE (stem‐expressed effector) genes were selected and placed under the control of the AvrLm4‐7 promoter, an effector gene highly expressed at the cotyledon stage of infection, for miniaturized cotyledon inoculation test screening of a gene pool of 204 rapeseed genotypes. We identified a rapeseed genotype, ‘Yudal’, expressing hypersensitive response to LmSTEE98. The LmSTEE98–RlmSTEE98 interaction was further validated by inactivation of the LmSTEE98 gene with a CRISPR‐Cas9 approach. Isolates with mutated versions of LmSTEE98 induced more severe stem symptoms than the wild‐type isolate in ‘Yudal’. This single‐gene resistance was mapped in a 0.6 cM interval of the ‘Darmor_bzh’ × ‘Yudal’ genetic map. One typical gene‐for‐gene interaction contributes partly to quantitative resistance when L. maculans colonizes the stems of rapeseed. With numerous other effectors specific to stem colonization, our study provides a new route for resistance gene discovery, elucidation of quantitative resistance mechanisms and selection for durable resistance. See also the Commentary on this article by Veneault‐Fourrey & Rep, 231: 1301–1303.
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影响因子：
5.4
作者：
Ghanbarnia, Kaveh;Lydiate, Derek J.;Fernando, W. G. Dilantha
通讯作者：
Fernando, W. G. Dilantha
影响因子：
3.7
作者：
Huang, Yong-Ju;Qi, Aiming;Fitt, Bruce D. L.
通讯作者：
Fitt, Bruce D. L.
影响因子：
4.4
作者：
Delourme R;Falentin C;Fomeju BF;Boillot M;Lassalle G;André I;Duarte J;Gauthier V;Lucante N;Marty A;Pauchon M;Pichon JP;Ribière N;Trotoux G;Blanchard P;Rivière N;Martinant JP;Pauquet J
通讯作者：
Pauquet J
DOI：
10.1007/s00122-015-2620-z
发表时间：
2016-01
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Huang YJ;Jestin C;Welham SJ;King GJ;Manzanares-Dauleux MJ;Fitt BD;Delourme R
通讯作者：
Delourme R
影响因子：
2.7
作者：
Fitt, B. D. L.;Hu, B. C.;White, R. P.
通讯作者：
White, R. P.