Development of cytidine 5'-monophosphate-protected gold-nanoclusters to be a direct luminescent substrate via aggregation-induced emission enhancement for ratiometric determination of alkaline phosphatase and inhibitor evaluation
Development of cytidine 5'-monophosphate-protected gold-nanoclusters to be a direct luminescent substrate via aggregation-induced emission enhancement for ratiometric determination of alkaline phosphatase and inhibitor evaluation
复制标题
DOI：
10.1016/j.colsurfa.2022.128423
复制
发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Yuqing Wu
中科院分区：
文献类型：
--
作者：
Chun-Xia Zhang;Yu Wang;Xinhe Duan;Kai Chen;Hong-Wei Li;Yuqing Wu
5‘-单磷酸保护的金纳米团簇(AuNCs@CMP)是一种荧光纳米底物，可用于碱性磷酸酶(ALP)的直接比例法测定。结合方式研究表明，配体CMP通过核糖和胞嘧啶两个氧原子与金核结合，而将磷酸基团自由地留在外面，促使AuNCs@CMP成为碱性磷酸酶的直接纳米底物。AuNCs@CMP在570 nm处发射，而AuNCs@Cyt的水解物在485 nm处发射，两者之间的大发射间隙为ALP的测定提供了一种比率计量法。此外，还引入了一种天然的、具有生物功能的低聚物--壳寡糖，它显著放大了AuNC@Cyt的荧光信号，将检测限提高到0.00026 U⋅L−1。此外，我们还深入研究了碱性磷酸酶对AuNCs@Cyt的水解和COS对AuNCs@Cyt的放大作用的内在机理，结果表明，这种发射增强是由于生成的AuNCs@Cyt具有聚集诱导发射增强(AIEE)特性。最后，将所建立的方法成功地应用于人血清中碱性磷酸酶的测定和碱性磷酸酶抑制剂的评价。因此，本研究开发了一种新的测定碱性磷酸酶的纳米底物，其范围为0.0050-0.25%
The cytidine 5′.-monophosphate (CMP)-protected gold nanoclusters (AuNCs@CMP) was developed to be a .ffuorescent nanosubstrate for direct ratiometric determination of alkaline phosphatase (ALP). The binding mode .investigations revealed that the ligand CMP binds to the gold core via two oxygen atoms of ribose and cytosine .and leaves the phosphate group freely outside, promoting AuNCs@CMP to be the direct nanosubstrate of ALP. .AuNCs@CMP emits at 570 nm while the hydrolysis product of AuNCs@Cyt emits at 485 nm; the large emission .gap between them provided a ratiometric approach for ALP determination. Moreover, a natural and biofunctional.oligomer, chitosan oligosaccharide (COS), was introduced to the system, which signiffcantly ampliffed .the ffuorescence signals of AuNCs@Cyt and improved the limit of detection (LOD) down to 0.00026 U⋅L− 1.. .Furthermore, the intrinsic mechanism of AuNCs@CMP hydrolysis by ALP and the COS ampliffcation on .AuNCs@Cyt were studied in-depth, which indicated the emission enhancement was attributed to the .aggregation-induced emission enhancement (AIEE) property of the produced AuNCs@Cyt. Finally, the developed .approach was successfully applied to determine ALP in human serum and the evaluation of ALP inhibitors. .Therefore, the present study develops a novel nanosubstrate for ALP determination in a range of 0.0050–0.25
登录
查看更多内容
使用 3-氨基苯硼酸功能化金纳米簇超灵敏检测碱性磷酸酶活性
DOI：
10.1016/j.snb.2018.10.083
发表时间：
2019-02
期刊：
Sensors and Actuators B: Chemical
影响因子：
--
作者：
Liu Qing;Li Hongxia;Jin Rui;Li Ning;Yan Xu;Su Xingguang
通讯作者：
Su Xingguang
影响因子：
9.3
作者：
Zhang Y;Zhou C;Li J;Zhang Y;Xie D;Liang M;Wang B;Song Y;Wang X;Huo Y;Hou FF;Xu X;Qin X
通讯作者：
Qin X
影响因子：
3
作者：
E. Mornet;F. Muller;S. Ngo;A. Taillandier;B. Simon‐Bouy;I. Maire;J. Oury
通讯作者：
E. Mornet;F. Muller;S. Ngo;A. Taillandier;B. Simon‐Bouy;I. Maire;J. Oury
一种新型可切换荧光传感器，可利用金/银纳米团簇轻松、高灵敏度地检测水环境中的碱性磷酸酶活性
影响因子：
4.3
作者：
Wang Xiaoyan;Liu Zhenjiang;Zhao Wanying;Sun Jianfan;Qian Bin;Wang Xinwei;Zeng Huawei;Du Daolin;Duan Jinsheng
通讯作者：
Duan Jinsheng
影响因子：
3.8
作者：
Lim SM;Kim YN;Park KH;Kang B;Chon HJ;Kim C;Kim JH;Rha SY
通讯作者：
Rha SY