A novel switchable fluorescent sensor for facile and highly sensitive detection of alkaline phosphatase activity in a water environment with gold/silver nanoclusters
A novel switchable fluorescent sensor for facile and highly sensitive detection of alkaline phosphatase activity in a water environment with gold/silver nanoclusters
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一种新型可切换荧光传感器，可利用金/银纳米团簇轻松、高灵敏度地检测水环境中的碱性磷酸酶活性
DOI：
10.1007/s00216-018-1514-8
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发表时间：
2018-12
影响因子：
4.3
通讯作者：
Duan Jinsheng
中科院分区：
文献类型：
--
作者：
Wang Xiaoyan;Liu Zhenjiang;Zhao Wanying;Sun Jianfan;Qian Bin;Wang Xinwei;Zeng Huawei;Du Daolin;Duan Jinsheng
开发了一种基于牛血清白蛋白稳定的金/银纳米簇 (BSA-Au/Ag NC) 的新型荧光传感器，用于灵敏、简便地检测碱性磷酸酶 (ALP) 活性。对于这种荧光传感器，抗坏血酸 2-磷酸 (AAP) 在 ALP 催化下分解为抗坏血酸 (AA) 和磷酸盐。 BSA-Au/Ag NCs 的初始红色荧光被 KMnO4 有效猝灭，然后通过添加 AA 恢复荧光。利用荧光寿命和紫外-可见吸收光谱研究了BSA-Au/Ag NCs与KMnO4和AA之间的相互作用机制。结果表明，KMnO4/AA 调节的氧化/还原导致 BSA-Au/Ag NC 表面结构破坏/恢复，从而导致荧光猝灭/恢复。所提出的基于暗背景的荧光方法用于检测ALP，具有优异的灵敏度，检测限为0.00076 U/L。此外，该方法应用于添加分析物的测定，回收率令人满意（97.0-105.0%）。该方法在模拟富营养化水体中成功检测出实际水样中的ALP，并可通过目视观察监测ALP活性的动态变化。更重要的是，所提出的荧光传感器不仅具有操作简单、灵敏度高的优点，而且还成功地应用于滤纸上，建立了快速、可视的ALP试纸。
A novel fluorescent sensor based on bovine serum albumin stabilized gold/silver nanoclusters (BSA-Au/Ag NCs) was developed for sensitive and facile detection of alkaline phosphatase (ALP) activity. For this fluorescent sensor, ascorbic acid 2-phosphate (AAP) was decomposed into ascorbic acid (AA) and phosphate by catalysis with ALP. The initial red fluorescence of the BSA-Au/Ag NCs was effectively quenched by KMnO4and then the fluorescence was recovered by addition of AA. The mechanism of interaction between BSA-Au/Ag NCs and KMnO4and AA was studied with use of the fluorescence lifetime and UV-vis absorption spectra. The results indicated that the oxidation/reduction modulated by KMnO4/AA led to surface structure destruction/restoration of the BSA-Au/Ag NCs, resulting in fluorescence quenching/recovery. The proposed fluorescence-based method based on a dark background was used to detect ALP and had excellent sensitivity, with a detection limit of 0.00076 U/L. Moreover, the method was applied to the determination of added analytes, with satisfactory recoveries (97.0–105.0 %). In a simulated eutrophic water body, this method successfully detected ALP in actual water samples and could monitor the dynamic changes of ALP activity through visual observation. More importantly, the proposed fluorescent sensor not only has the advantages of simple operation and high sensitivity but has also been successfully used on filter paper to establish a rapid and visual test paper for ALP.
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影响因子：
2.6
作者：
Charles Rose;R. Axler
通讯作者：
Charles Rose;R. Axler
影响因子：
9.7
作者：
A. Vivas;A. Marulanda;M. Gómez;J. Barea;R. Azcón
通讯作者：
A. Vivas;A. Marulanda;M. Gómez;J. Barea;R. Azcón
影响因子：
9.3
作者：
E. Schoenau;K. Herzog;H. Boehles
通讯作者：
E. Schoenau;K. Herzog;H. Boehles
基于四苯乙烯分子聚集和解聚集的碱性磷酸酶和抑制剂筛选的新型荧光启动测定
影响因子：
4.2
作者：
Gu, Xinggui;Zhang, Guanxin;Zhang, Deqing
通讯作者：
Zhang, Deqing
影响因子：
2.7
作者：
GAGE, MA;GORHAM, E
通讯作者：
GORHAM, E