Anatomy and ontogeny of a novel hemodynamic organ in zebrafish.
Anatomy and ontogeny of a novel hemodynamic organ in zebrafish.
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DOI：
10.1002/ar.23002
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发表时间：
2014-12
影响因子：
2
通讯作者：
LeClair, Elizabeth E.
中科院分区：
文献类型：
--
作者：
Binelli, Erica A.;Luna, Alejandra N.;LeClair, Elizabeth E.
斑马鱼的上颌须可以对刺激作出反应而伸长和缩回，并且似乎与上颌骨侧面的突出血窦相连。然而，没有描述触须运动的机制。使用全封鬼笔环肽染色的窦区，我们观察到长丝状肌动蛋白电缆，表明高度组织化的血管平滑肌细胞，周围的内皮腔。虽然红细胞在体内充满了腔室，但心脏注射荧光葡聚糖表明它始终含有血浆。全层共聚焦成像的葡聚糖注射的成人含有EGFP+内皮细胞显示了血管复合物的三个隔间，这里命名为远端球，中央室，和辅助室。所有三个隔间的早期个体发育确认在一个整体安装系列的Tg（fli1a：EGFP）青少年。在野生型成人，每个室的精细结构进行了研究，石蜡和塑料切片组织化学和透射电子显微镜。远端球和中央腔室有平滑肌被，腔室间隔延长，形成半分离的充满血液的腔隙。通过乙酰化微管蛋白（轴突细胞质的一种成分）的免疫组织化学检测证实，中央室壁和间隔广泛受小的无髓鞘轴突的神经支配。副腔既无神经支配，也无肌肉化，而是一个内皮盲囊，有增厚的弹性外膜，表明有一个可扩展的液体储存器。我们提出，我们已经在斑马鱼中发现了一个新器官，即上颌触须血窦，其神经血管专门化可能有助于斑马鱼的感觉生物学和附属物控制。
The zebrafish maxillary barbel can protract and retract in response to stimuli, and appears connected to a prominent blood sinus on the lateral aspect of the maxillary bone. However, the mechanism of barbel movement is not described. Using whole-mount phalloidin staining of the sinus region, we observed long filamentous actin cables, suggesting highly organized vascular smooth muscle cells, surrounding an endothelial chamber. Although the chamber is variably filled by erythrocytes in vivo, cardiac injection of fluorescent dextrans shows that it consistently contains plasma. Full-thickness confocal imaging of dextran-injected adults containing EGFP+ endothelial cells revealed a vascular complex with three compartments, here named the distal bulb, central chamber, and accessory chamber. The early ontogeny of all three compartments was confirmed in a whole-mount series of Tg(fli1a:EGFP) juveniles. In wild type adults, the fine structure of each chamber was studied using paraffin- and plastic-section histochemistry and transmission electron microscopy. The distal bulb and central chamber have smooth muscle coats with luminally-elongated septa, forming semi-detached blood-filled lacunae. The central chamber walls and septa are extensively innervated by small, unmyelinated axons, as confirmed by immunohistochemical detection of acetylated tubulin, a component of axonal cytoplasm. The accessory chamber appears neither innervated nor muscularized, but is an endothelial cul-de-sac with a thickened elastic adventitia, suggesting an extensible fluid reservoir. We propose that we have identified a new organ in zebrafish, the maxillary barbel blood sinus, whose neurovascular specializations may contribute to zebrafish sensory biology and appendage control.
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影响因子：
2
作者：
Adriaens, D;Verraes, W
通讯作者：
Verraes, W
DOI：
10.1111/dgd.12035
发表时间：
2013-02
期刊：
Development, growth & differentiation
影响因子：
--
作者：
Duszynski RJ;Topczewski J;LeClair EE
通讯作者：
LeClair EE
影响因子：
1.7
作者：
Hyvarinen, H.;Palviainen, A.;Holopainen, I. J.
通讯作者：
Holopainen, I. J.
DOI：
10.1002/ar.a.20273
发表时间：
2006-01-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
Marshall, CD;Amin, H;Lydersen, C
通讯作者：
Lydersen, C
影响因子：
3.6
作者：
Maklad, A;Fritzsch, B;Hansen, LA
通讯作者：
Hansen, LA