Super-Resolution Ultrasound Localization Microscopy on a Rabbit Liver VX2 Tumor Model: An Initial Feasibility Study.
Super-Resolution Ultrasound Localization Microscopy on a Rabbit Liver VX2 Tumor Model: An Initial Feasibility Study.
复制标题
DOI：
10.1016/j.ultrasmedbio.2021.04.012
复制
发表时间：
2021-08
影响因子：
2.9
通讯作者：
Song P
中科院分区：
文献类型：
--
作者：
Zhang W;Lowerison MR;Dong Z;Miller RJ;Keller KA;Song P
超声定位显微镜可以在不牺牲成像穿透深度的情况下对体内微血管进行成像。然而，对超分辨率推断的依赖限制了该技术的适用性，因为亚像素组织运动会破坏微血管重建。因此，大多数先前关于该超分辨率过程的临床前研究已被限制为具有充分运动校正和/或数据拒绝的低运动实验模型，这排除了具有表现出高度呼吸和其他运动的器官部位的成像。在本文中，我们提出了一种新的麻醉协议，诱导安全可控的呼吸暂停期，使超声定位显微镜所需的长成像采集时间的兔子。我们将此方案应用于接受索拉非尼治疗的VX 2肝肿瘤模型，并将结果与常规高剂量异氟烷麻醉的超分辨率图像进行比较。我们发现，呼吸暂停协议是必要的，以正确识别血管化不良的肿瘤核心，通过免疫组织化学验证，并揭示肿瘤微血管结构。
Ultrasound localization microscopy can image microvasculature in vivo without sacrificing imaging penetration depth. However, the reliance on super-resolution inference limits the applicability of the technique as subpixel tissue motion can corrupt microvascular reconstruction. Consequently, the majority of previous pre-clinical research on this super-resolution procedure has been restricted to low motion experimental models with ample motion correction and/or data rejection, which precludes the imaging of organ sites with that exhibit a high degree of respiratory and other motion. In this paper, we present a novel anesthesia protocol in rabbits that induces safe controllable periods of apnea to enable the long imaging acquisition times required for ultrasound localization microscopy. We apply this protocol to a VX2 liver tumor model undergoing sorafenib therapy and compare the results to super-resolution images from a conventional high-dose isoflurane anesthesia. We found that the apneic protocol was necessary to correctly identify the poorly vascularized tumor cores, as verified by immunohistochemistry, and to reveal the tumoral microvascular architecture.
登录
查看更多内容
影响因子：
4.6
作者：
Foiret J;Zhang H;Ilovitsh T;Mahakian L;Tam S;Ferrara KW
通讯作者：
Ferrara KW
影响因子：
4.2
作者：
Hingot, Vincent;Errico, Claudia;Couture, Olivier
通讯作者：
Couture, Olivier
影响因子：
158.5
作者：
Llovet, Josep M.;Ricci, Sergio;Bruix, Jordi
通讯作者：
Bruix, Jordi
DOI：
10.1109/tuffc.2020.2988159
发表时间：
2020-09-01
影响因子：
3.6
作者：
Lowerison, Matthew R.;Huang, Chengwu;Song, Pengfei
通讯作者：
Song, Pengfei
影响因子：
10.6
作者：
Christensen-Jeffries, Kirsten;Browning, Richard J.;Eckersley, Robert J.
通讯作者：
Eckersley, Robert J.