DNA nanotechnology in the undergraduate laboratory: Analysis of molecular topology using DNA nanoswitches.
DNA nanotechnology in the undergraduate laboratory: Analysis of molecular topology using DNA nanoswitches.
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DOI：
10.1021/acs.jchemed.9b01185
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发表时间：
2020-05-12
影响因子：
3
通讯作者：
Chandrasekaran AR
中科院分区：
文献类型：
--
作者：
Punnoose JA;Halvorsen K;Chandrasekaran AR
在学术实验室进行的前沿研究（如纳米技术）与本科实验室教授的内容之间存在脱节。在目前的本科课程中，很少有学生有机会在纳米技术相关的实验中做动手实验，其中大部分是通过选择性的本科研究计划。在大多数情况下，复杂的合成程序，昂贵的试剂，并要求特定的仪器防止广泛适应纳米技术为基础的实验室课程。DNA是一种纳米级分子，最近已被用于自下而上的纳米技术，应用于传感、纳米机器人和计算。在这篇文章中，我们提出了一个简单的实验，涉及DNA纳米开关的合成，可以改变其形状从一个线性的“关”状态的目标DNA分子的存在下，一个环状的“开”状态。实验还展示了纳米开关的环状状态的可编程拓扑结构及其对凝胶迁移的影响。该实验很容易在本科实验室中适应，仅需要琼脂糖凝胶电泳，材料设置成本最低，并且可以在3小时内完成。
There is a disconnect between the cutting-edge research done in academic labs, such as nanotechnology, and what is taught in undergraduate labs. In the current undergraduate curriculum, very few students get a chance to do hands-on experiments in nanotechnology-related experiments most of which are through selective undergraduate research programs. In most cases, complicated synthesis procedures, expensive reagents, and requirement of specific instrumentation prevent broad adaptation of nanotechnology-based experiments to laboratory courses. DNA, being a nanoscale molecule, has recently been used in bottom-up nanotechnology with applications in sensing, nano-robotics, and computing. In this article, we propose a simple experiment involving the synthesis of a DNA nanoswitch that can change its shape from a linear “off” state to a looped “on” state in the presence of a target DNA molecule. The experiment also demonstrates the programmable topology of the looped state of the nanoswitch and its effect on gel migration. The experiment is easy to adapt in an undergraduate laboratory, requires only agarose gel electrophoresis, a minimal set-up cost for materials, and can be completed in a 3-hour time frame.
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