Requirement for CD4+ T Cells in the Friend Murine Retrovirus Neutralizing Antibody Response: Evidence for Functional T Cells in Genetic Low-Recovery Mice
Requirement for CD4+ T Cells in the Friend Murine Retrovirus Neutralizing Antibody Response: Evidence for Functional T Cells in Genetic Low-Recovery Mice
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Friend 鼠逆转录病毒中和抗体反应对 CD4 T 细胞的需求：遗传低恢复小鼠功能性 T 细胞的证据
作者：
H. Super;Diane Brooks;K. Hasenkrug;B. Chesebro
摘要从感染Friend小鼠白血病逆转录病毒复合体(FV)中恢复需要T辅助细胞和细胞毒性T细胞以及中和抗体。几个宿主基因，包括主要组织相容性复合体(H-2)基因和Anh-2非连锁基因Rfv-3，影响这些FV特异性免疫反应。(B10.A×A/Wy)F1小鼠具有H-2a/aRfv-3r/S基因型，不能产生可检测到的Fv特异性T细胞增殖反应，但仍能产生Fv特异性中和免疫球蛋白M抗体，并能消除Fv病毒血症。因此，这种主要受Rfv-3基因影响的IgM反应可能是T细胞无关的。为了验证这一想法，在体内，小鼠体内的CD4+或CD8+T细胞群被耗尽，并监测了FV感染后对中和抗体反应的影响。令人惊讶的是，与未耗尽或CD8耗尽的小鼠相比，CD4+细胞耗尽的小鼠表现出无法检测到的Fv中和抗体反应和高病毒血症水平。除了敲除FV抗体反应外，CD4+T细胞耗尽显著缩短了生存时间，进一步表明了CD4+T细胞的重要性。这些研究揭示了这些低恢复小鼠感染FV后功能T细胞反应的第一个证据，并表明Rfv-3控制的FV抗体反应需要CD4+T辅助细胞。
ABSTRACT Recovery from infection with the Friend murine leukemia retrovirus complex (FV) requires T-helper cells and cytotoxic T cells as well as neutralizing antibodies. Several host genes, including genes of the major histocompatibility complex (H-2) and anH-2-unlinked gene, Rfv-3, influence these FV-specific immune responses. (B10.A × A/Wy)F1mice, which have the H-2a/aRfv-3r/s genotype, fail to mount a detectable FV-specific T-cell proliferative response but nevertheless produce FV-specific neutralizing immunoglobulin M (IgM) antibodies and can eliminate FV viremia. Thus, this IgM response, primarily influenced by the Rfv-3 gene, may be T-cell independent. To test this idea, mice were depleted of either CD4+ or CD8+ T-cell populations in vivo and were monitored for the effect on the neutralizing antibody response following FV infection. Surprisingly, mice in which CD4+ cells were depleted showed undetectable FV-neutralizing antibody responses and high viremia levels compared to nondepleted or CD8-depleted animals. In addition to knocking out the FV antibody response, CD4+ T-cell depletion reduced survival time significantly, further indicating the importance of CD4+ T cells. These studies revealed the first evidence for a functional T-cell response following FV infection in these low-recovery mice and showed that CD4+ T-helper cells are required for the Rfv-3-controlled FV antibody response.
细胞毒性 T 淋巴细胞在传染病中的作用：历史、标准和最新技术。
影响因子：
--
作者：
Oldstone,MB
通讯作者：
Oldstone,MB
影响因子：
56.9
作者：
Rosenberg, ES;Billingsley, JM;Walker, BD
通讯作者：
Walker, BD
影响因子：
158.5
作者：
COREY, L;SPEAR, PG
通讯作者：
SPEAR, PG