SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts
SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts
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SAGA1和SAGA2促进拟南芥叶绿体中原核蛋白周围淀粉的形成
DOI：
10.1101/2023.11.25.568654
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Atkinson N
中科院分区：
文献类型：
--
作者：
Atkinson N
类蛋白核是一种叶绿体微区室，大多数藻类和一些陆生植物在其中浓缩初级羧化酶 Rubisco（1,5-二磷酸核酮糖羧化酶/加氧酶），作为 CO2 浓缩机制的一部分，提高 CO2 捕获效率。将基于蛋白核的二氧化碳浓缩机制 (pCCM) 设计到 C3 作物中是提高产量能力和对气候变化的适应能力的一种有前景的策略。许多蛋白核的特征是淀粉板鞘，被认为可以作为限制二氧化碳扩散的屏障。最近，我们在模型 C3 植物拟南芥中重建了相分离的“原类核”Rubisco 基质，使用来自研究最充分的核类的藻类莱茵衣藻 [N.阿特金森，Y. Mao，K. X. Chan，A. J. McCormick，Nat。公报.11, 6303 (2020)]。在这里，我们描述了引入衣藻蛋白 StArch Granules Abnormal 1 (SAGA1) 和 SAGA2 的影响，它们与类核淀粉生物发生和形态的调节有关。我们发现SAGA1定位于工程拟南芥植物中的原核蛋白，这导致形成包围在原核蛋白凝聚物内的非典型球形淀粉颗粒和部分覆盖凝聚物的相邻板状颗粒，但不改变所产生的叶绿体淀粉的总量。 SAGA2的额外表达进一步增加了合成为完全包围原核蛋白的相邻板状颗粒的淀粉的比例。我们的研究结果为在维管植物中组装扩散屏障作为功能性 pCCM 的一部分铺平了道路，同时也增进了我们对 SAGA1 和 SAGA2 在淀粉鞘形成中的作用的理解，并拓宽了工程淀粉形态的途径。
The pyrenoid is a chloroplastic microcompartment in which most algae and some terrestrial plants condense the primary carboxylase, Rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase) as part of a CO2-concentrating mechanism that improves the efficiency of CO2capture. Engineering a pyrenoid-based CO2-concentrating mechanism (pCCM) into C3 crop plants is a promising strategy to enhance yield capacities and resilience to the changing climate. Many pyrenoids are characterized by a sheath of starch plates that is proposed to act as a barrier to limit CO2diffusion. Recently, we have reconstituted a phase-separated “proto-pyrenoid” Rubisco matrix in the model C3 plantArabidopsis thalianausing proteins from the alga with the most well-studied pyrenoid,Chlamydomonas reinhardtii[N. Atkinson, Y. Mao, K. X. Chan, A. J. McCormick,Nat. Commun.11, 6303 (2020)]. Here, we describe the impact of introducing the Chlamydomonas proteins StArch Granules Abnormal 1 (SAGA1) and SAGA2, which are associated with the regulation of pyrenoid starch biogenesis and morphology. We show that SAGA1 localizes to the proto-pyrenoid in engineered Arabidopsis plants, which results in the formation of atypical spherical starch granules enclosed within the proto-pyrenoid condensate and adjacent plate-like granules that partially cover the condensate, but without modifying the total amount of chloroplastic starch accrued. Additional expression of SAGA2 further increases the proportion of starch synthesized as adjacent plate-like granules that fully encircle the proto-pyrenoid. Our findings pave the way to assembling a diffusion barrier as part of a functional pCCM in vascular plants, while also advancing our understanding of the roles of SAGA1 and SAGA2 in starch sheath formation and broadening the avenues for engineering starch morphology.
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影响因子：
30.8
作者：
Fauser, Friedrich;Vilarrasa-Blasi, Josep;Onishi, Masayuki;Ramundo, Silvia;Patena, Weronika;Millican, Matthew;Osaki, Jacqueline;Philp, Charlotte;Nemeth, Matthew;Salome, Patrice A.;Li, Xiaobo;Wakao, Setsuko;Kim, Rick G.;Kaye, Yuval;Grossman, Arthur R.;Niyogi, Krishna K.;Merchant, Sabeeha S.;Cutler, Sean R.;Walter, Peter;Dinneny, Jose R.;Jonikas, Martin C.;Jinkerson, Robert E.
通讯作者：
Jinkerson, Robert E.
影响因子：
7.7
作者：
Zhang Y;Xu B;Weiner BG;Meir Y;Wingreen NS
通讯作者：
Wingreen NS
光合作用和新陈代谢中的生物分子凝聚体。
影响因子：
9.5
作者：
T. Wunder;O. Mueller
通讯作者：
O. Mueller
DOI：
10.1073/pnas.79.22.6903
发表时间：
1982-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HOWE, CJ;AUFFRET, AD;GRAY, JC
通讯作者：
GRAY, JC
影响因子：
3.7
作者：
Yu Zhan;C. Marchand;Alexandre Maes;Adeline Mauriès;Yi Sun;James S. Dhaliwal;J. Uniacke;Simon Arragain;Heng Jiang;Nicholas D. Gold;V. Martin;S. Lemaire;W. Zerges
通讯作者：
W. Zerges