Rapid, sensitive, and quantitative detection of pathogenic DNA at the point of care through microfluidic electrochemical quantitative loop-mediated isothermal amplification.

Rapid, sensitive, and quantitative detection of pathogenic DNA at the point of care through microfluidic electrochemical quantitative loop-mediated isothermal amplification.
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DOI:
10.1002/anie.201109115
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发表时间:
2012-05-14
影响因子:
16.6
通讯作者:
Soh, H. Tom
Soh, H. Tom
中科院分区:
化学1区
文献类型:
--
作者:
Hsieh, Kuangwen;Patterson, Adriana S.;Ferguson, B. Scott;Plaxco, Kevin W.;Soh, H. Tom

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我们提出了微流控电化学定量环介导等温扩增(MEQ-LAMP)平台,用于在护理点快速,灵敏和定量检测病原体基因组DNA。在单片微流体装置内以真实的时间电化学监测DNA扩增,使得能够在一小时内通过单步过程检测少至16个拷贝的沙门氏菌基因组DNA。
We present the Microfluidic Electrochemical Quantitative Loop-mediated isothermal AMPlification (MEQ-LAMP) platform for rapid, sensitive, and quantitative detection of pathogen genomic DNA at the point of care. DNA amplification is electrochemically monitored in real time within a monolithic microfluidic device, enabling the detection of as few as 16 copies of Salmonella genomic DNA via a single-step process in under an hour.
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