Expression and retinoic acid regulation of the zebrafish nr2f orphan nuclear receptor genes.
Expression and retinoic acid regulation of the zebrafish nr2f orphan nuclear receptor genes.
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DOI：
10.1002/dvdy.23838
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发表时间：
2012-10
影响因子：
2.5
通讯作者：
Prince, Victoria E.
中科院分区：
文献类型：
--
作者：
Love, Crystal E.;Prince, Victoria E.
关键词：
脊椎动物核受体亚家族2，f组（nr2f）基因编码孤儿受体，其具有充当视黄酸（RA）信号传导的负调节剂的能力。我们描述胚胎和幼虫的表达的六个斑马鱼nr2f基因，nr2f1a，nr2f1b，nr2f2和nr2f5。这些基因在中枢神经系统内表现出高度调节的表达模式，包括在发育中的后脑以及中胚层和内胚层中。我们还研究了RA和FGF信号在调节早期nr2f基因表达中的作用。RA是不需要的nr2f表达在后脑，然而，外源性RA可以抑制这种表达。相反，我们发现，RA正调控树干内胚层和中胚层nr2f1a的表达。Fgf信号不是nr2f在后脑表达所必需的，但是，它可能在维持菱体特异性表达中起作用。我们报告四个nr2f基因在所有三个胚层的详细表达分析。nr2f在后脑中表达的开始不需要RA或FGF信号。我们的发现，RA积极调节nr2f1a表达的躯干支持的可能性，Nr2fs功能的负反馈回路，以调节RA信号在这一地区。
The vertebrate nuclear receptor subfamily 2, group f (nr2f) genes encode orphan receptors that have the capacity to act as negative regulators of retinoic acid (RA) signaling. We describe embryonic and larval expression of four of the six zebrafish nr2f genes, nr2f1a, nr2f1b, nr2f2 and nr2f5. These genes show highly regulated patterns of expression within the CNS, including in the developing hindbrain, as well as in the mesoderm and endoderm. We also investigated the role of RA and Fgf signaling in regulating early nr2f gene expression. RA is not required for nr2f expression in the hindbrain; however, exogenous RA can repress this expression. Conversely, we find that RA positively regulates nr2f1a expression in trunk endoderm and mesoderm. Fgf signaling is not required for nr2f expression onset in the hindbrain; however, it may play a role in maintaining rhombomere-specific expression. We report detailed expression analysis of four nr2f genes in all three germ layers. The onset of nr2f expression in the hindbrain does not require RA or Fgf signals. Our finding that RA positively regulates nr2f1a expression in the trunk supports the possibility that Nr2fs function in a negative feedback loop to modulate RA signaling in this region.
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影响因子：
64.5
作者：
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影响因子：
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作者：
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通讯作者：
SCHILLING, TF