Establishing community-wide DNA barcode references for conserving mangrove forests in China.
Establishing community-wide DNA barcode references for conserving mangrove forests in China.
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建立全社区 DNA 条形码参考以保护中国红树林
DOI：
10.1186/s12870-021-03349-z
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发表时间：
2021-12-04
影响因子：
5.3
通讯作者：
Guo Z
中科院分区：
文献类型：
--
作者：
Mao X;Xie W;Li X;Shi S;Guo Z
红树林生态系统一直是全球关注的焦点，因为它们在保护沿海社区和通过封存“蓝碳”减缓全球气候变化方面发挥着至关重要的作用。中国的红树林多样性比较丰富，约占世界红树林的三分之一。70种真正的红树林物种和一些沿着该国海岸自然生长的红树林相关物种。然而，红树林生态系统受到日益加剧的人为干扰和海平面上升的广泛威胁。DNA条形码技术可以通过提供快速的物种识别来帮助保护红树林生态系统。为了研究这种潜力，在中国33个主要的红树林站点收集了898个植物标本。经形态学鉴定，共鉴定出72种红树植物，其中包括中国记录的28种真红树林和12种红树林伴生种。选择了三个叶绿体DNA标记rbcL，trnH-psbA，matK，和一个核标记ITS 2来研究使用条形码来鉴定这些物种的实用性。根据遗传距离、序列相似性和系统发育单系性的条形码缺口标准，我们认为ITS 2标记足以对中国红树植物及其近缘种进行条形码标记。此外，rbcL或trnH-psbA也可用于收集补充确认数据。在使用这些条形码，我们揭示了一个非常低的水平的遗传变异的地理位置之间的红树林物种，这是一个警报，他们对气候和人为干扰的脆弱性。建议利用ITS 2对华南地区的红树林物种和陆生海岸植物进行条形码化。我们获得的DNA条形码序列在监测生物多样性和生态系统恢复方面具有重要价值，这对红树林保护至关重要。在线版本包含补充材料，可通过10.1186/s12870-021-03349-z获得。
Mangrove ecosystems have been the focus of global attention for their crucial role in sheltering coastal communities and retarding global climate change by sequestering ‘blue carbon’. China is relatively rich in mangrove diversity, with one-third of the ca. 70 true mangrove species and a number of mangrove associate species occurring naturally along the country’s coasts. Mangrove ecosystems, however, are widely threatened by intensifying human disturbances and rising sea levels. DNA barcoding technology may help protect mangrove ecosystems by providing rapid species identification. To investigate this potential, 898 plant specimens were collected from 33 major mangrove sites in China. Based on the morphologic diagnosis, the specimens were assigned to 72 species, including all 28 true mangrove species and all 12 mangrove associate species recorded in China. Three chloroplast DNA markers rbcL, trnH-psbA, matK, and one nuclear marker ITS2 were chosen to investigate the utility of using barcoding to identify these species. According to the criteria of barcoding gaps in genetic distance, sequence similarity, and phylogenetic monophyly, we propose that a single marker, ITS2, is sufficient to barcode the species of mangroves and their associates in China. Furthermore, rbcL or trnH-psbA can also be used to gather supplement confirming data. In using these barcodes, we revealed a very low level of genetic variation among geographic locations in the mangrove species, which is an alert to their vulnerability to climate and anthropogenic disturbances. We suggest using ITS2 to barcode mangrove species and terrestrial coastal plants in South China. The DNA barcode sequences we obtained would be valuable in monitoring biodiversity and the restoration of ecosystems, which are essential for mangrove conservation. The online version contains supplementary material available at 10.1186/s12870-021-03349-z.
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影响因子：
10
作者：
Li, Xiwen;Yang, Yang;Chen, Shilin
通讯作者：
Chen, Shilin
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
7.7
作者：
Liu, Jinxin;Shi, Linchun;Downie, Stephen R.
通讯作者：
Downie, Stephen R.
影响因子：
6.5
作者：
Meier, Rudolf;Shiyang, Kwong;Ng, Peter K. L.
通讯作者：
Ng, Peter K. L.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro