Functional Analyses of the Bacillus velezensis HMB26553 Genome Provide Evidence That Its Genes Are Potentially Related to the Promotion of Plant Growth and Prevention of Cotton Rhizoctonia Damping-Off.
Functional Analyses of the Bacillus velezensis HMB26553 Genome Provide Evidence That Its Genes Are Potentially Related to the Promotion of Plant Growth and Prevention of Cotton Rhizoctonia Damping-Off.
复制标题
velezensis HMB26553基因组的功能分析为其基因可能与促进植物生长和预防棉花根丝核菌枯败有关提供了证据。
作者：
芽孢杆菌是一类具有代表性的防治植物病害和促进植物生长的生防制剂。本研究获得了细菌菌株HMB 26553的全基因组序列。基于基因组和平均核苷酸同源性（ANI）以及dDDH（digital DNA-DNA hybridization）构建系统发育树，将菌株HMB 26553鉴定为Velezensis芽孢杆菌。通过anti-SMASH预测了14个次级代谢产物的生物合成基因簇，并通过UHPLC-QTOF-MS/MS（超高效液相色谱-四极杆飞行时间串联质谱）鉴定了6种次级代谢产物。B. velezensis HMB 26553的菌丝结构发生变化，活性氧积累，线粒体膜电位降低。通过基因组信息和实验预测并证实了菌株HMB 26553的特性，如产生IAA、铁载体、胞外酶和生物膜，以及移动和促进棉花生长。这些结果提示了B. Velezensis HMB 26553对病原菌的生长有抑制作用，对棉花的生长有促进作用，可能为防治棉花立枯病提供了潜在的生防菌。
Bacillus spp. is one kind of the important representative biocontrol agents against plant diseases and promoting plant growth. In this study, the whole genomic sequence of bacterial strain HMB26553 was obtained. A phylogenetic tree based on the genome and ANI (average nucleotide identity), as well as dDDH (digital DNA–DNA hybridization), was constructed, and strain HMB26553 was identified as Bacillus velezensis. Fourteen biosynthetic gene clusters responsible for secondary metabolite were predicted via anti-SMASH, and six secondary metabolites were identified by UHPLC-QTOF-MS/MS (ultra-high-performance liquid chromatography coupled to quadrupole-time-of-flight tandem mass spectrometry). When the phytopathogen Rhizoctonia solani was treated with B. velezensis HMB26553, the mycelial structure changed, ROS (reactive oxygen species) accumulated, and the mitochondrial membrane potential decreased. Characteristics of strain HMB26553 were predicted and confirmed by genomic information and experiments, such as producing IAA, siderophore, extracellular enzymes and biofilm, as well as moving and promoting cotton growth. All these results suggested the mechanisms by which B. velezensis HMB26553 inhibits pathogen growth and promotes cotton growth, which likely provided the potential biocontrol agent to control cotton Rhizoctonia damping-off.
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影响因子：
4.1
作者：
Fira, Djordje;Dimkic, Ivica;Stankovic, Slavisa
通讯作者：
Stankovic, Slavisa
影响因子：
3.2
作者：
Guetsky, R;Shtienberg, D;Dinoor, A
通讯作者：
Dinoor, A
影响因子：
3.2
作者：
Howell, Charles R.
通讯作者：
Howell, Charles R.
从自然环境中分离的枯草芽孢杆菌对番茄枯萎病的生物防治取决于介导生物膜形成的保守基因。
影响因子：
5.1
作者：
Chen Y;Yan F;Chai Y;Liu H;Kolter R;Losick R;Guo JH
通讯作者：
Guo JH
影响因子：
4.5
作者：
Bacharis, C.;Gouziotis, A.;Karaoglanidis, G. S.
通讯作者：
Karaoglanidis, G. S.