Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation.
Biocontrol of tomato wilt disease by Bacillus subtilis isolates from natural environments depends on conserved genes mediating biofilm formation.
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从自然环境中分离的枯草芽孢杆菌对番茄枯萎病的生物防治取决于介导生物膜形成的保守基因。
DOI：
10.1111/j.1462-2920.2012.02860.x
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发表时间：
2013-03
影响因子：
5.1
通讯作者：
Guo JH
中科院分区：
文献类型：
--
作者：
Chen Y;Yan F;Chai Y;Liu H;Kolter R;Losick R;Guo JH
摘要枯草芽孢杆菌和其他芽孢杆菌长期以来一直被用作植物细菌性病害的生物防治剂，但细菌赋予保护作用的机制尚不清楚。本研究的目的是分离B菌株。枯草芽孢杆菌，表现出高水平的生物防治效力，从自然环境中，并调查这些菌株赋予植物保护的机制。从中国各地采集的60个菌株中筛选出6个在温室条件下对番茄青枯病菌（Ralstonia solanacearum）具有50%以上生防效果的菌株。这些野生菌株能够在确定的培养基和番茄植物根部形成强大的生物膜，并在平板测定中对各种植物病原体表现出强烈的拮抗活性。我们发现，这些菌株的植物保护依赖于生物膜形成所需的广泛保守的基因，包括调控基因和基质生产基因。我们提供的证据表明，基质的生产是至关重要的细菌定植在植物根表面。最后，我们建立了一个研究B的模型系统。枯草杆菌-番茄植物相互作用在抵抗植物病原体的保护中的作用。
SummaryBacillus subtilis and other Bacilli have long been used as biological control agents against plant bacterial diseases but the mechanisms by which the bacteria confer protection are not well understood. Our goal in this study was to isolate strains of B. subtilis that exhibit high levels of biocontrol efficacy from natural environments and to investigate the mechanisms by which these strains confer plant protection. We screened a total of 60 isolates collected from various locations across China and obtained six strains that exhibited above 50% biocontrol efficacy on tomato plants against the plant pathogen Ralstonia solanacearum under greenhouse conditions. These wild strains were able to form robust biofilms both in defined medium and on tomato plant roots and exhibited strong antagonistic activities against various plant pathogens in plate assays. We show that plant protection by those strains depended on widely conserved genes required for biofilm formation, including regulatory genes and genes for matrix production. We provide evidence suggesting that matrix production is critical for bacterial colonization on plant root surfaces. Finally, we have established a model system for studies of B. subtilis–tomato plant interactions in protection against a plant pathogen.
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影响因子：
64.5
作者：
BURBULYS, D;TRACH, KA;HOCH, JA
通讯作者：
HOCH, JA
影响因子：
11.4
作者：
Chai, Yunrong;Norman, Thomas;Losick, Richard
通讯作者：
Losick, Richard
影响因子：
3.6
作者：
Hamon, MA;Lazazzera, BA
通讯作者：
Lazazzera, BA
影响因子：
4.2
作者：
Aliye, Naser;Fininsa, Chemeda;Hiskias, Yaynu
通讯作者：
Hiskias, Yaynu
DOI：
10.1073/pnas.0610503104
发表时间：
2007-01-30
影响因子：
11.1
作者：
Butcher, Rebecca A.;Schroeder, Frank C.;Clardy, Jon
通讯作者：
Clardy, Jon