Establishment of two midgut cell lines from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)
Establishment of two midgut cell lines from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae)
复制标题
秋粘虫、草地贪夜蛾（鳞翅目：夜蛾科）的两个中肠细胞系的建立
DOI：
10.1007/s11626-019-00420-w
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Stanley, David
中科院分区：
文献类型：
--
作者：
Zhou, Kaile;Goodman, Cynthia L.;Ringbauer, Joseph;Song, Qisheng;Beerntsen, Brenda;Stanley, David
从夜蛾幼虫的内脏中获得了两个细胞系，并进行了26次传代50多次。CT/ bcirl - sfmg -0611- kz系由27个胰蛋白酶化、切碎的整个中肠组织建立；CT/BCIRL-SfMG-0617-KZ系来自分离的28个中肠肌肉组织（含残余上皮细胞）。另外29个中肠培养物来自分离的上皮细胞；有的传代不超过3次，生长缓慢，存活时间超过1年。连续复制的细胞系包含31个具有不同形态的牢固粘附细胞，包括细长，球形和/或32个矩形。这些细胞系的平均直径分别为9.3±4.0 μm （SfMG1-0611）和9.2±3.9 33 μm （SfMG-0617）。SfMG1-0611和SfMG-0617的生长曲线具有较长的倍增时间，分别为73.9 34 h和50.4 h。利用DNA扩增指纹图谱（DAF-PCR）对35个细胞系进行鉴定，发现其中36个细胞系的DNA模式与宿主组织相似，但与其他细胞系或37个不同昆虫种的组织存在明显差异。用38个物种特异性引物扩增基因组DNA，得到了预期大小的DNA片段，序列与来自这些引物的39个片段几乎相同。frugiperdagenome。两种细胞系暴露于选定的dbtcry蛋白40中，影响最小。目前，全世界的研究人员都可以使用这些细胞系。
Two cell lines were generated from larval midguts ofSpodoptera frugiperdaand have been 26 passaged over 50 times. The CT/BCIRL-SfMG1-0611-KZ line was established from 27 trypsinized, minced whole midgut tissues: the CT/BCIRL-SfMG-0617-KZ line from isolated 28 midgut muscle tissue (containing some residual epithelial cells). Additional midgut cultures were 29 generated from isolated epithelial cells; some passaged not more than three times, which grew 30 very slowly and survived longer than 1 year. The continuously replicating cell lines contain 31 firmly adhering cells with different morphologies, including elongated, spherical, and/or 32 rectangular. The mean diameters of these cell lines are 9.3 ± 4.0 μm (SfMG1-0611) and 9.2 ± 3.9 33 μm (SfMG-0617). Growth curves for the two lines have relatively lengthy doubling times of 73.9 34 h and 50.4 h for SfMG1-0611 and SfMG-0617, respectively. We confirmed the identity of these 35 lines using DNA amplification fingerprinting (DAF-PCR) and noted that the DNA patterns for 36 each cell line were similar to their host tissues but distinctly different from other cell lines or 37 tissues from different insect species. Amplification of genomic DNA with species-specific 38 primers yielded DNA fragments of the expected sizes and with sequences nearly identical to 39 those from theS. frugiperdagenome. Both cell lines were exposed to selectedBtCry proteins 40 with minimal impact. These lines are currently available to researchers worldwide.
登录
查看更多内容
DOI：
10.1007/s11626-015-9870-6
发表时间：
2015-02
期刊：
In Vitro Cellular & Developmental Biology - Animal
影响因子：
--
作者：
Jianghua Li;Fei He;Yongbo Yang;Yutao Xiao;Rong Peng;H. Yao;Xianchun Li;Jianxin Peng;H. Hong;Kaiyu Liu
通讯作者：
Jianghua Li;Fei He;Yongbo Yang;Yutao Xiao;Rong Peng;H. Yao;Xianchun Li;Jianxin Peng;H. Hong;Kaiyu Liu
来自 Pseudaletia unipuncta（鳞翅目：夜蛾科）的原代和连续中肠细胞培养物
DOI：
--
发表时间：
2001
期刊：
In Vitro Cellular & Developmental Biology - Animal
影响因子：
--
作者：
J. García;Guo‐xun Li;Ping Wang;J. Zhong;R. Granados
通讯作者：
R. Granados
影响因子：
3.7
作者：
Goergen G;Kumar PL;Sankung SB;Togola A;Tamò M
通讯作者：
Tamò M
使用杆状病毒表达载体系统在大规模搅拌生物反应器中生产重组蛋白。
影响因子：
--
作者：
Christine M Thompson;Johnny Montes;M. Aucoin;A. Kamen
通讯作者：
A. Kamen
鳞翅目幼虫中肠上皮细胞原代和连续培养物的建立
DOI：
--
发表时间：
1994
期刊：
影响因子：
--
作者：
D. Baines;A. Brownwright;J. Schwartz
通讯作者：
J. Schwartz