Primary and continuous midgut cell cultures fromPseudaletia unipuncta (lepidoptera: Noctuidae)
Primary and continuous midgut cell cultures fromPseudaletia unipuncta (lepidoptera: Noctuidae)
复制标题
来自 Pseudaletia unipuncta（鳞翅目：夜蛾科）的原代和连续中肠细胞培养物
DOI：
--
复制
发表时间：
2001
期刊：
影响因子：
--
通讯作者：
R. Granados
中科院分区：
文献类型：
--
作者：
J. García;Guo‐xun Li;Ping Wang;J. Zhong;R. Granados
本研究采用胶原酶消化法从五龄单斑拟谷盗幼虫的中肠组织中分离出中肠上皮细胞，并在TNM-FH培养基中进行培养。用P.单点粘虫肠细胞。从这些细胞中获得了几个细胞系。unipuncta原代培养物，并已传代培养和维持超过24个月。培养物中存在三种主要的中肠细胞类型，包括干细胞（再生）、柱状细胞和杯状细胞。观察干细胞体外形态发生及向柱状细胞和杯状细胞的分化。每7-8周杯状细胞和柱状细胞出现一个细胞死亡周期，然后由干细胞替代。在大约6次传代后，T瓶中的细胞密度似乎有些恒定，达到每毫升培养基103-104个细胞。柱状细胞为圆形至矩形，具有刷状缘，而杯状细胞具有典型的烧瓶状形状，具有中央空腔。在所有培养瓶中均观察到围食膜样分泌物。这些细胞与多重嵌入的核型多角体病毒的感染得到证实，我们的结论是，这些中肠细胞可以作为一个体外模型系统，研究杆状病毒感染的早期事件。
SummaryMidgut epithelial cells were isolated from fifth-instarPseudaletia unipuncta larvae by collagenase treatment of midgut tissue, and cultured in TNM-FH medium. Long-term continuous culture and maintenance of midgut cells were achieved withP. unipuncta armyworm intestinal cells. Several cells lines were obtained from theseP. unipuncta primary cultures, and they have been subcultured and maintained for over 24 mo. The three major midgut cell types were present in the cultures, including stem (regenerative), columnar, and goblet cells. In vitro morphogenesis and differentiation of columnar and goblet cells from stem cells were observed. There appeared to be a cycle of cell death of goblet and columnar cells followed by their replacement from stem cells every 7–8 wk. After approximately six passages, the cell density in T-flasks appeared to be somewhat constant, reaching 103–104 cells per milliliter of medium. The columnar cells are round to rectangular in shape and possess a brush border, while the goblet cells have a classic flask-like shape with a central cavity. Peritrophic membrane-like secretions were observed in all the culture flasks. Infection of these cells with multiply embedded nucleopolyhedrovirus was confirmed, and we conclude that these midgut cells can be used as an in vitro model system to study early events in baculovirus infection.
viking：果蝇中第二种 IV 型胶原蛋白的鉴定和表征。
影响因子：
3.5
作者：
Yasothornsrikul,S;Davis,WJ;Cramer,G;Kimbrell,DA;Dearolf,CR
通讯作者：
Dearolf,CR
影响因子：
11.4
作者：
KUSCHEGULLBERG, M;GARRISON, K;FESSLER, JH
通讯作者：
FESSLER, JH