Molecular characterization reveals that OsSAPK3 improves drought tolerance and grain yield in rice.
Molecular characterization reveals that OsSAPK3 improves drought tolerance and grain yield in rice.
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分子鉴定表明OsSAPK3提高了水稻的耐旱性和产量。
DOI：
10.1186/s12870-023-04071-8
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发表时间：
2023-01-24
影响因子：
5.3
通讯作者：
Yang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Lou, Dengji;Lu, Suping;Chen, Zhen;Lin, Yi;Yu, Diqiu;Yang, Xiaoyan
许多资料表明，蔗糖非发酵1-相关激酶2（SnRK 2）在植物非生物胁迫中起着重要作用。在水稻中，这些激酶被称为渗透胁迫/ABA活化蛋白激酶（SAPKs）。渗透胁迫/ABA活化蛋白激酶3（OsSAPK 3）是水稻SnRK 2 Ⅱ的成员，但其功能尚不清楚。干旱、NaCl、PEG和阿坝均上调OsSAPK 3的表达。OsSAPK 3突变体sapk 3 -1和sapk 3 -2对外源阿坝的敏感性降低。此外，在干旱条件下，sapk 3 -1和sapk 3 -2表现出更强的耐旱性，包括存活率降低、失水率增加、气孔导度增加以及SLAC 1和SLAC 7的表达水平显著降低。生理和代谢分析表明，OsSAPK 3可能通过影响渗透调节和渗透调节物质、ROS解毒以及阿坝依赖和非依赖脱水反应基因的表达，在干旱胁迫信号转导中发挥重要作用。所有农艺性状分析表明，OsSAPK 3可以通过影响分蘖数和籽粒大小的调节来提高水稻产量。OsSAPK 3在ABA依赖和ABA非依赖的干旱胁迫响应中均发挥重要作用。更有趣的是，OsSAPK 3可以通过间接调节分蘖数和籽粒大小来提高水稻产量。这些发现为抗旱水稻的发展提供了新的思路。在线版本包含补充材料，可通过10.1186/s12870-023-04071-8获得。
Many data suggest that the sucrose non-fermenting 1-related kinases 2 (SnRK2s) are very important to abiotic stress for plants. In rice, these kinases are known as osmotic stress/ABA–activated protein kinases (SAPKs). Osmotic stress/ABA–activated protein kinase 3 (OsSAPK3) is a member of SnRK2II in rice, but its function is still unclear. The expression of OsSAPK3 was up regulated by drought, NaCl, PEG and ABA. OsSAPK3 mutated seedings (sapk3-1 and sapk3-2) showed reduced hypersensitivity to exogenous ABA. In addition, under drought conditions, sapk3-1 and sapk3-2 showed more intolerance to drought, including decreased survival rate, increased water loss rate, increased stomatal conductance and significantly decreased expression levels of SLAC1 and SLAC7. Physiological and metabolic analyses showed that OsSAPK3 might play an important role in drought stress signaling pathway by affecting osmotic adjustment and osmolytes, ROS detoxification and expression of ABA dependent and independent dehydration-responsive genes. All gronomic traits analyses demonstrated that OsSAPK3 could improve rice yield by affecting the regulation of tiller numbers and grain size. OsSAPK3 plays an important role in both ABA-dependent and ABA-independent drought stress responses. More interestingly, OsSAPK3 could improve rice yield by indirectly regulating tiller number and grain size. These findings provide new insight for the development of drought-resistant rice. The online version contains supplementary material available at 10.1186/s12870-023-04071-8.
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