Type VI collagen in the rat testis: monoclonal antibody, isolation, and localization during development.
Type VI collagen in the rat testis: monoclonal antibody, isolation, and localization during development.
复制标题
大鼠睾丸中的 VI 型胶原蛋白：单克隆抗体、分离和发育过程中的定位。
作者：
Hajime Sawada;F. Yazama
为了研究生精小管细胞外基质 (ST-ECM) 的成分，产生了针对 ST-ECM 的单克隆抗体。其中之一 (15B6) 识别出 VI 型胶原蛋白的非还原形式。使用该抗体作为探针，从 ST-ECM 的 SDS-尿素提取物中分离出 VI 型胶原蛋白。它呈四聚体形式，还原后解离成一个主要的 140 kDa 带以及两个 190 和 210 kDa 的带。通过免疫荧光显微镜研究了 VI 型胶原分布的发育变化。在 16 天的大鼠胚胎中，当肌样细胞开始分化（VI 型）时，在泌尿生殖肠系膜中可以看到胶原蛋白，并且它已经开始包围性腺，在某些胚胎中还包围生精索。在新生大鼠中，在脐带之间的细胞外间隙中发现了它，位于间质中。然而，在成人中，它仅限于生精小管周围的两条平行线和血管系统外膜中的丝状结构。免疫电镜显示，这种胶原独立于胶原原纤维分布在间质中，但被排除在支持细胞的基底层之外。这些观察结果表明，VI 型胶原蛋白是 ST-ECM 的主要成分，并且它对于基质的组织和管周细胞的分化可能很重要。
To investigate the components of the seminiferous tubule extracellular matrix (ST-ECM), monoclonal antibodies were raised against ST-ECM. One of these (15B6) recognized the nonreduced form of type VI collagen. With this antibody as a probe, type VI collagen was isolated from an SDS-urea extract of ST-ECM. It took a tetrameric form, and upon reduction dissociated into a major 140-kDa band and two bands at 190 and 210 kDa. Developmental changes in the distribution of type VI collagen were investigated by immunofluorescence microscopy. In 16-day rat embryos when myoid cells began to differentiate, type VI, collagen was seen in the urogenital mesentery, and it had begun to surround the gonad and, in some embryos, the seminiferous cords. In newborn rats, it was found throughout the extracellular spaces between the cords, being localized in the interstitium. In adults, however, it was confined to two parallel lines around the seminiferous tubules and to filamentous structures in the adventitia of vascular systems. Immunoelectron microscopy showed that this collagen was distributed in the interstitium independently from collagenous fibrils, but was excluded from the basal lamina of Sertoli cells. These observations indicate that type VI collagen is a major component of the ST-ECM and that it may be important in the organization of the matrix and in the differentiation of peritubular cells.
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影响因子：
3.6
作者：
Lian,G;Miller,KA;Enders,GC
通讯作者：
Enders,GC
人类 α 3(VI) 胶原蛋白基因。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Stokes,DG;Saitta,B;Timpl,R;Chu,ML
通讯作者：
Chu,ML
影响因子：
3.7
作者：
DOANE, KJ;YANG, G;BIRK, DE
通讯作者：
BIRK, DE
影响因子：
--
作者：
RUSSELL, LD;BARTKE, A;GOH, JC
通讯作者：
GOH, JC
影响因子：
2.9
作者：
BLAKE, MS;JOHNSTON, KH;GOTSCHLICH, EC
通讯作者：
GOTSCHLICH, EC