Generation of a murine monoclonal antibody to normal mammary epithelium using mice rendered immune-tolerant to malignant mammary epithelium.
Generation of a murine monoclonal antibody to normal mammary epithelium using mice rendered immune-tolerant to malignant mammary epithelium.
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使用对恶性乳腺上皮具有免疫耐受性的小鼠，产生针对正常乳腺上皮的鼠单克隆抗体。
DOI：
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发表时间：
1994
影响因子：
3.2
通讯作者：
C. Taylor
中科院分区：
文献类型：
--
作者：
S. Imam;E. Esteban;L. Young;C. Taylor
使用免疫前免疫耐受的程序产生了一种单克隆抗体（MAb），可区分组织或细胞系中的正常乳腺上皮和恶性乳腺上皮。在出生 24 小时内诱导新生小鼠对两种转化乳腺上皮细胞系（MCF.7 和 MDA.MB.231 细胞系组合）的免疫耐受。免疫耐受的成功诱导是通过间接免疫组织学方法确定的，该方法针对耐受原（即 MCF.7 和 MDA.MB.231 细胞系）测试小鼠血清。随后用正常乳腺上皮提取物对血清中缺乏抗免疫耐受原抗体的小鼠进行免疫。根据间接免疫组织学方法测定的血清抗体显示与正常乳腺上皮细胞（MEC）反应但不与侵袭性乳腺癌细胞反应的证据，选择一只小鼠用于杂交瘤生产。将所选小鼠的脾细胞与小鼠骨髓瘤细胞系融合以产生 MAb。经过广泛筛选后，根据与组织中正常 MEC 的反应性以及组织或致瘤 MEC 系中不存在恶性 MEC 染色的情况，对一种 MAb 进行了进一步研究。这项反应特异性测试表明，特异性抗体检测到的抗原在正常腺上皮的顶端质膜上表达，包括乳腺、子宫颈、结肠、肺、胰腺和胃，但在组织切片中的恶性对应物上不表达。 MAb 识别的抗原称为管腔上皮抗原，表观分子量为 92 KD (LEA.92)。这项研究说明了免疫耐受/免疫方法在产生具有特定特异性要求的抗体方面的实际用途，例如在鉴定在两种组织（例如正常组织和恶性组织）中差异表达的抗原时，否则这两种组织具有多种共同抗原。
A monoclonal antibody (MAb) that distinguishes normal from malignant mammary epithelia in tissue or cell lines was generated using a procedure that involved immune-tolerization before immunization. Immune-tolerance to two transformed mammary epithelial cell lines (MCF.7 and MDA.MB.231 cell lines combined) was induced in neonatal mice within 24 hr of birth. Successful induction of immune-tolerance was determined by an indirect immunohistological method, testing sera from mice against the tolerogen (i.e., the MCF.7 and MDA.MB.231 cell lines). Mice lacking antibodies in their sera against the immune-tolerogen were subsequently immunized with an extract of normal breast epithelium. One mouse was selected for hybridoma production based on evidence of serum antibody that showed reactivity with normal mammary epithelial cells (MEC) but not with invasive breast carcinoma cells, as determined by an indirect immunohistological method. Spleen cells from the selected mouse were fused with a mouse myeloma cell line to generate MAb. After extensive screening, one MAb was further studied on the basis of reactivity with normal MEC in tissue and absence of staining of malignant MEC in tissue or tumorigenic MEC lines. This test of specificity of reactivity revealed that the antigen detected by the specific antibody was expressed on the apical plasma membrane of normal glandular epithelia that included breast, cervix, colon, lung, pancreas, and stomach, but not on their malignant counterparts in tissue sections. The antigen recognized by the MAb was termed luminal epithelial antigen with an apparent MW of 92 KD (LEA.92). This study illustrates the practical usefulness of the immune-tolerization/immunization approach in the generation of antibodies with particular specificity requirements, as in the identification of an antigen that is differentially expressed in two tissues (e.g., normal and malignant) which otherwise have a multiplicity of antigens in common.
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影响因子：
11.2
作者：
B. Atkinson;C. Ernst;B. Ghrist;M. Herlyn;M. Błaszczyk;A. Ross;D. Herlyn;Z. Steplewski;H. Koprowski
通讯作者：
B. Atkinson;C. Ernst;B. Ghrist;M. Herlyn;M. Błaszczyk;A. Ross;D. Herlyn;Z. Steplewski;H. Koprowski
新生儿对磷酸胆碱的耐受引起的独特型转变。
DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Quintans,J;Quan,ZS
通讯作者：
Quan,ZS
新生儿 B 细胞耐受诱导的膜和代谢要求。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Teale,JM;Klinman,NR
通讯作者：
Klinman,NR
影响因子：
56.9
作者：
NOSSAL, GJV
通讯作者：
NOSSAL, GJV
单克隆抗体定义的人肺细胞表面蛋白抗原。
影响因子：
11.2
作者：
Mazauric,T;Mitchell,KF;Letchworth3rd,GJ;Koprowski,H;Steplewski,Z
通讯作者：
Steplewski,Z