Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene
Interaction of C/EBPbeta and v-Myb is required for synergistic activation of the mim-1 gene
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mim-1 基因的协同激活需要 C/EBPbeta 和 v-Myb 的相互作用
DOI：
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发表时间：
1996
影响因子：
5.3
通讯作者：
K. Klempnauer
中科院分区：
文献类型：
--
作者：
S. Mink;U. Kerber;K. Klempnauer
逆转录病毒癌基因v-myb编码转录因子（v-Myb），其通过与C/EBP转录因子家族的成员合作来激活骨髓单核细胞特异性mim-1基因（天然myb靶基因）。发现v-Myb与C/EBP一起足以激活异源细胞类型中的mim-1基因，这暗示Myb和C/EBP作为双向分子开关，其调节骨髓单核细胞特异性基因的表达。为了更详细地了解v-Myb和C/EBP之间的关系，我们研究了v-Myb和C/EBP β激活mim-1启动子的分子基础，C/EBP β是在骨髓单核细胞中高度表达的C/EBP转录因子家族的成员。我们已经确定了一个复合Myb和C/EBP的反应元件，介导的mim-1启动子的协同激活这两个因素，并组成紧密间隔的Myb和C/EBP结合位点。体外和体内蛋白结合研究表明，v-Myb和C/EBP β通过其DNA结合结构域相互作用。我们发现，这种相互作用是必不可少的mim-1启动子的v-Myb和C/EBPbeta的协同激活。因此，我们的工作确定C/EBPbeta作为参与骨髓单核细胞基因表达的v-Myb的相互作用伙伴。
The retroviral oncogene v-myb encodes a transcription factor (v-Myb) which activates the myelomonocyte-specific mim-1 gene, a natural myb target gene, by cooperating with members of the C/EBP transcription factor family. The finding that v-Myb, together with C/EBP, is sufficient to activate the mim-1 gene in heterologous cell types has implicated Myb and C/EBP as a bipartite molecular switch, which regulates the expression of myelomonocyte-specific genes. To understand the relationship between v-Myb and C/EBP in more detail, we have examined the molecular basis of the activation of the mim-1 promoter by v-Myb and C/EBPbeta, a member of the C/EBP transcription factor family highly expressed in myelomonocytic cells. We have identified a composite Myb and C/EBP response element which mediates synergistic activation of the mim-1 promoter by both factors and consists of closely spaced Myb- and C/EBP-binding sites. In vitro and in vivo protein-binding studies indicate that v-Myb and C/EBPbeta interact with each other via their DNA-binding domains. We show that this interaction is essential for the synergistic activation of the mim-1 promoter by v-Myb and C/EBPbeta. Our work therefore identifies C/EBPbeta as an interaction partner of v-Myb involved in myelomonocyte gene expression.
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影响因子：
64.5
作者：
Kathleen Weston;J.Michael Bishop
通讯作者：
Kathleen Weston;J.Michael Bishop
影响因子：
2.7
作者：
S. Nordeen
通讯作者：
S. Nordeen
DOI：
10.1073/pnas.89.17.8145
发表时间：
1992-09-01
影响因子：
11.1
作者：
LECLAIR, KP;BLANAR, MA;SHARP, PA
通讯作者：
SHARP, PA
影响因子：
56.9
作者：
GEWIRTZ, AM;CALABRETTA, B
通讯作者：
CALABRETTA, B
影响因子：
10.5
作者：
METZ, R;ZIFF, E
通讯作者：
ZIFF, E