Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.).
Development of a new marker system for identifying the complex members of the low-molecular-weight glutenin subunit gene family in bread wheat (Triticum aestivum L.).
复制标题
DOI：
10.1007/s00122-011-1550-7
复制
发表时间：
2011-05
影响因子：
5.4
通讯作者：
Zhang, Aimin
中科院分区：
文献类型：
--
作者：
Zhang, Xiaofei;Liu, Dongcheng;Yang, Wenlong;Liu, Kunfan;Sun, Jiazhu;Guo, Xiaoli;Li, Yiwen;Wang, Daowen;Ling, Hongqing;Zhang, Aimin
低分子量谷蛋白亚基（LMW-GSs）对面包小麦的面包品质起着重要的决定作用。然而，由于低分子量小麦- gs具有多基因编码的高多态性蛋白复合物，因此对低分子量小麦- gs复杂基因家族的研究仍具有挑战性。本研究利用传统的保守引物聚合酶链反应（PCR）和高分辨率毛细管电泳技术，建立了一种新的LMW-GS基因家族成员鉴定分子标记体系。在对中国面包小麦品种小偃54中已鉴定的13个LMW-GS基因和GenBank中其他基因进行序列比对的基础上，开发了PCR引物，并对LMW-GS基因长度多态性区域的保守序列进行了定位。PCR扩增后，用毛细管电泳法检测了小燕54的17个DNA片段。其中13个片段与先前鉴定的LMW-GS基因相同，另外4个片段通过测序来源于LMW-GS独特基因。该标记系统还用于中国春及其1组零四染色体系的LMW-GS基因的鉴定。在检测到的17个DNA片段中，4个位于1A染色体上，5个位于1B染色体上，8个位于1D染色体上。结果表明，该标记系统可用于面包小麦品种中低分子量- gs基因的大规模鉴定，并可用于小麦分子育种中选择所需的低分子量- gs基因以提高面包品质。本文的在线版本（doi:10.1007/s00122-011-1550-7）包含补充材料，仅供授权用户使用。
Low-molecular-weight glutenin subunits (LMW-GSs) play an important role in determining the bread-making quality of bread wheat. However, LMW-GSs display high polymorphic protein complexes encoded by multiple genes, and elucidating the complex LMW-GS gene family in bread wheat remains challenging. In the present study, using conventional polymerase chain reaction (PCR) with conserved primers and high-resolution capillary electrophoresis, we developed a new molecular marker system for identifying LMW-GS gene family members. Based on sequence alignment of 13 LMW-GS genes previously identified in the Chinese bread wheat variety Xiaoyan 54 and other genes available in GenBank, PCR primers were developed and assigned to conserved sequences spanning the length polymorphism regions of LMW-GS genes. After PCR amplification, 17 DNA fragments in Xiaoyan 54 were detected using capillary electrophoresis. In total, 13 fragments were identical to previously identified LMW-GS genes, and the other 4 were derived from unique LMW-GS genes by sequencing. This marker system was also used to identify LMW-GS genes in Chinese Spring and its group 1 nulli–tetrasomic lines. Among the 17 detected DNA fragments, 4 were located on chromosome 1A, 5 on 1B, and 8 on 1D. The results suggest that this marker system is useful for large-scale identification of LMW-GS genes in bread wheat varieties, and for the selection of desirable LMW-GS genes to improve the bread-making quality in wheat molecular breeding programmes. The online version of this article (doi:10.1007/s00122-011-1550-7) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
7.4
作者：
Masci, S;D'Ovidio, R;Kasarda, DD
通讯作者：
Kasarda, DD
影响因子：
2
作者：
DOvidio, R;Porceddu, E
通讯作者：
Porceddu, E
影响因子：
2.3
作者：
Mamone, Gianfranco;De Caro, Salvatore;Ferranti, Pasquale
通讯作者：
Ferranti, Pasquale
影响因子：
3.7
作者：
Dong L;Zhang X;Liu D;Fan H;Sun J;Zhang Z;Qin H;Li B;Hao S;Li Z;Wang D;Zhang A;Ling HQ
通讯作者：
Ling HQ
影响因子：
6.9
作者：
Balmer, Y;Vensel, WH;Hurkman, WJ
通讯作者：
Hurkman, WJ