Encystation of Entamoeba histolytica in Axenic Culture.
Encystation of Entamoeba histolytica in Axenic Culture.
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DOI：
10.3390/microorganisms9040873
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发表时间：
2021-04-18
期刊：
影响因子：
4.5
通讯作者：
Ingram-Smith C
中科院分区：
文献类型：
--
作者：
Wesel J;Shuman J;Bastuzel I;Dickerson J;Ingram-Smith C
溶组织内阿米巴是一种引起阿米巴痢疾的寄生性原生动物，全世界每年约有9000万人感染。E.溶组织菌是通过摄入被包囊形式污染的食物和水传播的，包囊形式在小肠中经历脱囊，变成定植在大肠中的滋养体形式。由于缺乏可重复的包囊形成，入侵内阿米巴是研究滋养体和包囊之间阶段转换的模型。在实验室中进行组织溶解。尽管人们对E. invadens，研究结果并不完全转化为E。由于这些物种之间存在广泛的遗传和宿主差异，在这里，我们提出了第一个可重复的包囊E。体外溶组织。产生的孢囊是可行的，并显示出四个特征性标志：圆形，几丁质细胞壁，四核，和洗涤剂抗性。流式细胞仪分析表明，葡萄糖限制和高细胞密度是包囊形成的关键，而E.入侵。短链脂肪酸乙酸酯和丙酸酯增强了进入包囊的能力，这与E。入侵。这一新模型将为进一步研究E.溶组织病阶段转换、传播和治疗。
Entamoeba histolytica is a parasitic protozoan that causes amoebic dysentery, which affects approximately 90 million people each year worldwide. E. histolytica is transmitted through ingestion of food and water contaminated with the cyst form, which undergoes excystation in the small intestine to the trophozoite form that colonizes the large intestine. The reptile pathogen Entamoeba invadens has served as a model for studying stage conversion between the trophozoite and cyst form due to lack of reproducible encystation of E. histolytica in the laboratory. Although much has been learned about encystation and excystation using E. invadens, the findings do not fully translate to E. histolytica due to the extensive genetic and host differences between these species. Here, we present the first reproducible encystation of E. histolytica in vitro. The cysts produced were viable and displayed the four characteristic hallmarks: round shape, chitinous cell wall, tetranucleation, and detergent resistance. Using flow cytometry analysis, glucose limitation and high cell density were key for encystation, as for E. invadens. Entry into encystation was enhanced by the short-chain fatty acids acetate and propionate, unlike for E. invadens. This new model will now allow the further study of E. histolytica stage conversion, transmission, and treatment.
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影响因子：
6.7
作者：
Chatterjee, Anirban;Ghosh, Sudip K.;Samuelson, John
通讯作者：
Samuelson, John
影响因子：
2.5
作者：
Campos-Góngora, E;Ebert, F;Tannich, E
通讯作者：
Tannich, E
影响因子：
3.8
作者：
Ali IK;Haque R;Siddique A;Kabir M;Sherman NE;Gray SA;Cangelosi GA;Petri WA Jr
通讯作者：
Petri WA Jr
影响因子：
2.1
作者：
Barron-Gonzalez, M. P.;Villarreal-Trevino, L.;Morales-Vallarta, M. R.
通讯作者：
Morales-Vallarta, M. R.
影响因子：
1.3
作者：
ARROYOBEGOVICH, A;CARABEZTREJO, A
通讯作者：
CARABEZTREJO, A