Apparatus for investigating the reactions of soft-bodied invertebrates to controlled humidity gradients.
Apparatus for investigating the reactions of soft-bodied invertebrates to controlled humidity gradients.
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DOI：
10.1016/j.jneumeth.2014.08.021
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发表时间：
2014-11-30
影响因子：
3
通讯作者：
Pierce-Shimomura, Jonathan T.
中科院分区：
文献类型：
--
作者：
Russell, Joshua;Pierce-Shimomura, Jonathan T.
虽然许多研究已经分析了动物对化学，温度和光梯度的行为反应，但很少有研究分析动物对湿度梯度的反应。我们的新的湿度箱，使我们能够研究线虫的湿度感觉的神经分子基础。我们描述了一个易于建造，低成本的湿度箱，以测定小动物的行为，包括软体无脊椎动物，在受控的湿度梯度。我们表明，我们的湿度室的设计是适合软体无脊椎动物，并能产生可靠的梯度范围为0.3- 8%RH/cm的9厘米长× 7.5厘米宽的凝胶覆盖的竞技场。以前的湿度室依赖于循环干燥和潮湿的空气，以在小的竞技场中产生陡峭的湿度梯度。为了消除可能引起独立于湿度响应的机械响应的移动空气的混淆，我们的室使用吸湿材料的储存器来控制湿度梯度。此外，为了更好地观察湿度响应的行为机制，我们的室提供了更大的竞技场。尽管之前已经描述了类似的腔室，但这些方法不适合软体无脊椎动物或容易的行为成像，因为它们需要动物穿过金属丝或织物网。我们的湿度箱的普遍适用性克服了以前设计的局限性，并打开了观察软体无脊椎动物行为反应的大门，包括遗传上强大的C。线虫和果蝇幼虫。
While many studies have assayed behavioral responses of animals to chemical, temperature and light gradients, fewer studies have assayed how animals respond to humidity gradients. Our novel humidity chamber has allowed us to study the neuromolecular basis of humidity sensation in the nematode Caenorhabditis elegans. We describe an easy-to-construct, low-cost humidity chamber to assay the behavior of small animals, including soft-bodied invertebrates, in controlled humidity gradients. We show that our humidity-chamber design is amenable to soft-bodied invertebrates and can produce reliable gradients ranging 0.3–8% RH/cm across a 9-cm long x 7.5-cm wide gel-covered arena. Previous humidity chambers relied on circulating dry and moist air to produce a steep humidity gradient in a small arena (e.g.). To remove the confound of moving air that may elicit mechanical responses independent of humidity responses, our chamber controlled the humidity gradient using reservoirs of hygroscopic materials. Additionally, to better observe the behavioral mechanisms for humidity responses, our chamber provided a larger arena. Although similar chambers have been described previously, these approaches were not suitable for soft-bodied invertebrates or for easy imaging of behavior because they required that animals move across wire or fabric mesh. The general applicability of our humidity chamber overcomes limitations of previous designs and opens the door to observe the behavioral responses of soft-bodied invertebrates, including genetically powerful C. elegans and Drosophila larvae.
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影响因子：
2.5
作者：
WARBURG, MR
通讯作者：
WARBURG, MR
影响因子：
1.5
作者：
Yu, Hai-Ping;Shao, Lin;Li, Guo-Qing
通讯作者：
Li, Guo-Qing
DOI：
10.1017/s0007485300026158
发表时间：
1938-01-01
期刊：
BULL ENT RES
影响因子：
--
作者：
THOMSON, R. C. M.
通讯作者：
THOMSON, R. C. M.
DOI：
10.1073/pnas.70.3.817
发表时间：
1973-01-01
影响因子：
11.1
作者：
WARD, S
通讯作者：
WARD, S
影响因子：
1.9
作者：
SOLOMON, ME
通讯作者：
SOLOMON, ME