A transcription factor DAF-5 functions in Haemonchus contortus development.
A transcription factor DAF-5 functions in Haemonchus contortus development.
复制标题
转录因子 DAF-5 在捻转血矛线虫发育中发挥作用
DOI：
10.1186/s13071-021-05036-2
复制
发表时间：
2021-10-12
影响因子：
3.2
通讯作者：
Hu M
中科院分区：
文献类型：
--
作者：
Di W;Li F;He L;Wang C;Zhou C;Liu L;Ye L;Chen J;Hu M
Background
Abnormal dauer formation gene (daf-5), located downstream of the DAF-7 signalling pathway, mainly functions in dauer formation and reproductive processes in the free-living nematode Caenorhabditis elegans. Although the structure and function of daf-5 have been clarified in C. elegans, they still remain totally unknown in Haemonchus contortus, a socio-economically important parasitic nematode of gastric ruminants.
Methods
A homologue of daf-5, Hc-daf-5, and its inferred product (Hc-DAF-5) in H. contortus were identified and characterized in this study. Then the transcriptional profiles of Hc-daf-5 and the anatomical expression of Hc-DAF-5 in H. contortus were studied using an integrated molecular approach. RNA interference (RNAi) was performed to explore its function in transition from the exsheathed third-stage larvae (xL3s) to the fourth-stage larvae (L4s) in vitro. Finally, the interaction between Hc-DAF-5 and Hc-DAF-3 (a co-Smad) was detected by bimolecular fluorescence complementation (BiFc) in vitro.
Results
It was shown that Hc-DAF-5 was a member of the Sno/Ski superfamily. Hc-daf-5 was transcribed in all developmental stages of H. contortus, with significant upregulation in L3s. Native Hc-DAF-5 was localized in the reproductive organs, cuticle, and intestine via immunohistochemistry. RNAi revealed that specific small interfering RNAs (siRNAs) could retard xL3 development. In addition, the interaction between Hc-DAF-5 and Hc-DAF-3 indicated that the SDS box of Hc-DAF-5 was dispensable for the binding of Hc-DAF-5 to Hc-DAF-3, and the MH2 domain was the binding region between Hc-DAF-3 and Hc-DAF-5.
Conclusions
In summary, these findings show that Hc-daf-5 functions in the developmental processes of H. contortus, and this study is the first attempt to characterize the daf-5 gene in parasitic nematodes.
Graphical abstract
登录
查看更多内容
DOI：
10.1534/g3.115.018929
发表时间：
2015-06-24
期刊：
G3 (Bethesda, Md.)
影响因子：
--
作者：
Matthews BB;Dos Santos G;Crosby MA;Emmert DB;St Pierre SE;Gramates LS;Zhou P;Schroeder AJ;Falls K;Strelets V;Russo SM;Gelbart WM;FlyBase Consortium
通讯作者：
FlyBase Consortium
影响因子：
4.8
作者：
Di, Wenda;Gasser, Robin B.;Hu, Min
通讯作者：
Hu, Min
影响因子：
5.4
作者：
Eccles D;Chandler J;Camberis M;Henrissat B;Koren S;Le Gros G;Ewbank JJ
通讯作者：
Ewbank JJ
影响因子：
6.7
作者：
Ma, Guangxu;Wang, Tao;Gasser, Robin B.
通讯作者：
Gasser, Robin B.
影响因子：
56.9
作者：
Hoskins, Roger A.;Carlson, Joseph W.;Celniker, Susan E.
通讯作者：
Celniker, Susan E.