Ultraviolet-C light for treatment of Candida albicans burn infection in mice.
Ultraviolet-C light for treatment of Candida albicans burn infection in mice.
复制标题
DOI：
10.1111/j.1751-1097.2011.00886.x
复制
发表时间：
2011-03
影响因子：
3.3
通讯作者：
Hamblin MR
中科院分区：
文献类型：
--
作者：
Dai T;Kharkwal GB;Zhao J;St Denis TG;Wu Q;Xia Y;Huang L;Sharma SK;d'Enfert C;Hamblin MR
烧伤患者具有侵袭性真菌感染的高风险，侵袭性真菌感染是发病率、死亡率和因耐药真菌菌株的出现而加剧的相关费用的主要原因。在这项研究中，我们研究了使用UVC光（254-nm）治疗白色念珠菌感染的小鼠Ⅲ度烧伤。体外实验表明，UVC可以选择性地杀死致病酵母C。白色念珠菌与正常角质形成细胞系相比，以光暴露依赖性的方式。采用小鼠慢性C.在非致死性三度烧伤中发生了白色念珠菌感染。梭用Gaussia princeps荧光素酶基因的一个版本稳定转化白色念珠菌菌株，该版本允许对念珠菌的进展进行实时生物发光成像。白色念珠菌感染在第0天（感染后30分钟）进行单次暴露的UVC治疗，当2.92 J/cm 2 UVC被输送时，真菌发光平均损失2.16-log10-unit（99.2%），而感染后24小时UVC在6.48 J/cm 2后真菌发光减少1.94-log10-unit（95.8%）。统计分析表明，在第0天和第1天进行的UVC处理显着降低了感染烧伤的真菌生物负载。UVC被认为是上级局部抗真菌药物，制霉菌素霜。在正常小鼠皮肤上测试UVC，并且在6.48 J/cm 2后24小时未观察到大体损伤。DNA损伤（环丁烷嘧啶二聚体）在正常小鼠皮肤免疫荧光观察后，立即在6.48 J/cm 2的UVC曝光，但病变广泛修复后24小时UVC曝光。
Burn patients are at high risk of invasive fungal infections, which are a leading cause of morbidity, mortality, and related expense exacerbated by the emergence of drug resistant fungal strains. In this study, we investigated the use of UVC light (254-nm) for the treatment of Candida albicans infection in mouse third degree burns. In-vitro studies demonstrated that UVC could selectively kill the pathogenic yeast C. albicans compared to a normal keratinocyte cell line in a light exposure dependent manner. A mouse model of chronic C. albicans infection in non-lethal 3rd degree burns was developed. The C. albicans strain was stably transformed with a version of the Gaussia princeps luciferase gene that allowed real-time bioluminescence imaging of the progression of C. albicans infection. UVC treatment with a single exposure carried out on day 0 (30 minutes post-infection) gave an average 2.16-log10-unit (99.2%) loss of fungal luminescence when 2.92 J/cm2 UVC had been delivered, while UVC 24-hours post-infection gave 1.94-log10-unit (95.8%) reduction of fungal luminescence after 6.48 J/cm2. Statistical analysis demonstrated that UVC treatment carried out both on both day 0 and day 1 significantly reduced the fungal bioburden of infected burns. UVC was found to be superior to a topical antifungal drug, nystatin cream. UVC was tested on normal mouse skin and no gross damage was observed 24 hours after 6.48 J/cm2. DNA lesions (cyclobutane pyrimidine dimers) were observed by immunofluorescence in normal mouse skin immediately after a 6.48 J/cm2 UVC exposure, but the lesions were extensively repaired at 24-hours after UVC exposure.
登录
查看更多内容
影响因子：
2.7
作者：
Bak, Jimmy;Ladefoged, Soren D.;Gregersen, Annette
通讯作者：
Gregersen, Annette
影响因子：
2.7
作者：
Jett, BD;Hatter, KL;Gilmore, MS
通讯作者：
Gilmore, MS
影响因子：
2.7
作者：
Gilpin, DA
通讯作者：
Gilpin, DA
影响因子：
3.1
作者：
Enjalbert, Brice;Rachini, Anna;d'Enfert, Christophe
通讯作者：
d'Enfert, Christophe
影响因子：
9
作者：
Horvath, Edward E.;Murray, Clinton K.;Cancio, Leopoldo C.
通讯作者：
Cancio, Leopoldo C.