Expression of Periostin, homologous with an insect cell adhesion molecule, as a prognostic marker in non-small cell lung cancers.
Expression of Periostin, homologous with an insect cell adhesion molecule, as a prognostic marker in non-small cell lung cancers.
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DOI：
10.1111/j.1349-7006.2001.tb01174.x
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发表时间：
2001-08
期刊：
影响因子：
--
通讯作者：
Fujii Y
中科院分区：
文献类型：
--
作者：
Sasaki H;Lo KM;Chen LB;Auclair D;Nakashima Y;Moriyama S;Fukai I;Tam C;Loda M;Fujii Y
我们使用回文聚合酶链反应 (PCR) 驱动的 cDNA 差异显示技术从癌症组织中识别并分离出一个名为骨膜素的基因，并发现它在几种人类肿瘤中过度表达。我们试图通过逆转录酶 (RT)-PCR 分析确定骨膜素表达对非小细胞肺癌 (NSCLC) 患者临床结果的影响。 RNA原位杂交显示Periostin基因在肺癌组织肿瘤周边高表达，但在肿瘤内部不表达，提示Periostin的表达可能参与了肿瘤的侵袭过程。尽管一些配对的正常肺样本表现出弱表达，但在 50 个（49.0％）肿瘤样本中检测到骨膜素转录本。骨膜素基因表达与性别、N 或 T 状态之间没有关系。骨膜蛋白表达的NSCLC患者生存率显着低于骨膜蛋白不表达的NSCLC患者（P=0.0338）。
We used our palindromic polymerase chain reaction (PCR)‐driven cDNA differential display technique to identify and isolate a gene, designated periostin, from cancer tissues and found it to be overexpressed in several human tumors. We attempted to determine the influence of periostin expression on clinical outcome in patients with non‐small cell lung cancer (NSCLC) by reverse transcriptase (RT)‐PCR analysis. Periostin gene was highly expressed at the tumor periphery of lung cancer tissue but not within the tumor by in situ RNA hybridization, suggesting that expression of periostin may be involved in the process of tumor invasion. Periostin transcripts were detected in 50 (49.0%) of the tumor samples, although some paired normal lung samples showed weak expression. There was no relationship between periostin gene expression and gender, N‐ or T‐status. The NSCLC patients with periostin expression had significantly poorer survival than the patients without periostin expression (P=0.0338).
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影响因子：
64.5
作者：
ZINN, K;MCALLISTER, L;GOODMAN, CS
通讯作者：
GOODMAN, CS
影响因子：
168.9
作者：
HART, IR;SAINI, A
通讯作者：
SAINI, A
影响因子：
4.1
作者：
TAKESHITA, S;KIKUNO, R;AMANN, E
通讯作者：
AMANN, E
影响因子：
3.1
作者：
SKONIER, J;NEUBAUER, M;PURCHIO, AF
通讯作者：
PURCHIO, AF
影响因子：
9.6
作者：
Mountain, CF
通讯作者：
Mountain, CF