The distribution of DNA translocation times in solid-state nanopores.
The distribution of DNA translocation times in solid-state nanopores.
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DOI：
10.1088/0953-8984/22/45/454129
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发表时间：
2010-11-17
期刊：
影响因子：
--
通讯作者：
Talaga DS
中科院分区：
文献类型：
--
作者：
Li J;Talaga DS
本文系统地研究了溶液粘度、外加电压和DNA链长对DNA通过直径为8±2 nm的氮化硅纳米孔的移位时间分布的影响。基于电流阻断的幅度选择线性dsDNA易位事件，并累积到电流阻断和事件持续时间（易位时间）的散点图中。易位时间分布拟合的解决方案的Smoluchowski型方程的一维偏置扩散到一个水槽。从拟合中提取了DNA在偏压下的漂移速度和扩散常数作为溶液粘度、施加电压和DNA链长度的函数。结合Einstein-Smoluchowski关系，该模型允许评估DNA分子上的粘性阻力。该模型还允许估计的不确定性，在确定DNA链的长度，由于布朗运动的贡献。数据分析表明，简单的一维偏置扩散模型拟合的实验数据，以及为广泛的条件。观察到与预测行为的一些偏差，并显示额外的现象可能有助于DNA易位时间的分布。
This paper systematically investigates the effects of solution viscosity, applied voltage, and DNA chain length on the distribution of DNA translocation times through 8±2 nm diameter silicon nitride nanopores. Linear dsDNA translocation events were selected based on the magnitude of current blockage and accumulated into scatter plots of current blockage and event duration (translocation time). The translocation time distribution was fit to the solution of a Smoluchowski-type equation for 1D biased diffusion to a sink. The DNA drifting speed under bias and diffusion constant were extracted from the fits as functions of solution viscosity, applied voltage, and DNA chain length. Combined with the Einstein–Smoluchowski relation, this model allowed evaluation of the viscous drag force on DNA molecules. This model also allowed estimation of the uncertainty in determining the DNA chain length due to the contribution of Brownian motion. The data analysis suggests that the simple 1D biased diffusion model fits the experimental data well for a wide range of conditions. Some deviations from predicted behavior were observed and show where additional phenomena are likely to contribute to the distribution of DNA translocation times.
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