Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin.
Immunogenicity and protective efficacy against Treponema pallidum in New Zealand rabbits immunized with plasmid DNA encoding flagellin.
复制标题
编码鞭毛蛋白的质粒 DNA 免疫新西兰兔，其免疫原性和对梅毒螺旋体的保护作用
DOI：
10.1038/s41426-018-0176-0
复制
发表时间：
2018-11-07
影响因子：
13.2
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Zheng K;Xu M;Xiao Y;Luo H;Xie Y;Yu J;Tan M;Li Y;Zhao F;Zeng T;Wu Y
将编码鞭毛蛋白FlaB 3的质粒DNA用作疫苗接种候选物，用于评价免疫原性和针对梅毒螺旋体亚种的保护。苍白球播散首先，肌肉注射由质粒DNA编码的鞭毛蛋白到新西兰兔引起体液和细胞免疫应答。总IgG生产增加响应鞭毛蛋白。此外，鞭毛蛋白FlaB 3质粒免疫家兔后，血清IFN-γ分泌量和CD 8+细胞数量明显高于重组鞭毛蛋白免疫家兔。与对照组相比，质粒DNA编码的鞭毛蛋白诱导血清IL-6和IL-8显著上调。随后，用1 × 107 T对免疫新西兰兔进行皮内攻击。在鞭毛蛋白质粒DNA接种的兔子中，苍白球导致血液、肝脏、脾脏和睾丸中细菌器官负荷的显著降低。此外，组织病理学分析表明，用质粒DNA编码的鞭毛蛋白（FlaB 3）免疫的家兔具有更好的免疫保护作用。这些发现提供了证据，质粒DNA编码的鞭毛蛋白（FlaB 3）可能是有用的作为一个潜在的免疫途径，为未来的疫苗开发，以抑制T。相关动物的苍白球播散。
Plasmid DNA encoding flagellin FlaB3 was used as a vaccination candidate for the evaluation of immunogenicity and protection against Treponema pallidum subsp. pallidum dissemination. First, intramuscular injection of the flagellin encoded by the plasmid DNA into New Zealand rabbits elicited both humoral and cellular immune responses. Total IgG production increased in response to flagellin. In addition, serum IFN-γ secretion and CD8+ cells were substantially greater in the rabbits immunized with the plasmid encoding flagellin FlaB3 than those in the rabbits immunized with recombinant flagellin. The flagellin encoded by the plasmid DNA induced significant upregulation of serum IL-6 and IL-8 compared to that of the control rabbits. Subsequently, intradermal challenge of the vaccinated New Zealand rabbits with 1 × 107T. pallidum resulted in a significant reduction of the bacterial organ burden in the blood, liver, spleen, and testicles in the flagellin plasmid DNA-vaccinated rabbits. Furthermore, the histopathological analysis demonstrated that the rabbits immunized with the plasmid DNA-encoded flagellin (FlaB3) showed better immune protection. These findings provide evidence that plasmid DNA-encoded flagellin (FlaB3) may be useful as a potential immunization route for future development of a vaccine to inhibit T. pallidum dissemination in related animals.
登录
查看更多内容
DOI：
10.1073/pnas.0502040102
发表时间：
2005-06-28
影响因子：
11.1
作者：
Andersen-Nissen, E;Smith, KD;Aderem, A
通讯作者：
Aderem, A
影响因子：
4.4
作者：
Eaves-Pyles, T;Murthy, K;Salzman, AL
通讯作者：
Salzman, AL
影响因子：
--
作者：
Lukehart, Sheila A;Marra, Christina M
通讯作者：
Marra, Christina M
影响因子：
6.4
作者：
BAKERZANDER, SA;LUKEHART, SA
通讯作者：
LUKEHART, SA
影响因子：
3.1
作者：
McDermott, PF;Ciacci-Woolwine, F;Mizel, SB
通讯作者：
Mizel, SB