Biomimetic approach to articular cartilage tissue engineering using carbon nanotube-coated and textured polydimethylsiloxane scaffolds.
Biomimetic approach to articular cartilage tissue engineering using carbon nanotube-coated and textured polydimethylsiloxane scaffolds.
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DOI：
10.1111/nyas.14769
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发表时间：
2022-07
影响因子：
5.2
通讯作者：
Jurewicz, Izabela
中科院分区：
文献类型：
--
作者：
Elidottir, Katrin Lind;Scott, Louie;Lewis, Rebecca;Jurewicz, Izabela
有一个显着的需要，了解关节软骨的复杂性和异质性，以开发更有效的治疗策略，如骨关节炎的疾病。在这里，我们表明，碳纳米管（CNT）是一个很好的候选人作为合成支架材料，以支持软骨细胞的生长，细胞产生和维持软骨。软骨细胞的形态，增殖和对齐进行了研究，纳米级碳纳米管网络被施加到宏观纹理的聚二甲基硅氧烷（PDMS）支架。将CNT应用于基于PDMS的支架表面导致细胞粘附增加10倍，增殖增加240%，这归因于纳米级粗糙度和亲水性的增加。将宏观尺度特征引入PDMS诱导软骨细胞排列，成功地模拟了在软骨表层中观察到的细胞行为。使用拉曼光谱作为非侵入性、无标记方法来监测细胞外基质产生和软骨细胞表型。这些支架上的软骨细胞成功地产生胶原蛋白、糖胺聚糖和聚集蛋白聚糖。这项研究表明，引入不同长度尺度的物理特征允许对组织支架设计进行高水平的控制，从而控制细胞行为。最终，这些纹理支架可以作为平台，以提高对骨关节炎的理解和早期治疗测试。图形摘要：有一个显着的需要，了解关节软骨的复杂性和异质性，以开发更有效的治疗策略，如骨关节炎的疾病。在这里，我们表明，碳纳米管（CNT）是一个很好的候选人作为合成支架材料，以支持软骨细胞的生长。软骨细胞的形态，增殖和对齐进行了研究，纳米级碳纳米管网络被施加到宏观纹理的聚二甲基硅氧烷（PDMS）支架。
There is a significant need to understand the complexity and heterogeneity of articular cartilage to develop more effective therapeutic strategies for diseases such as osteoarthritis. Here, we show that carbon nanotubes (CNTs) are excellent candidates as a material for synthetic scaffolds to support the growth of chondrocytes—the cells that produce and maintain cartilage. Chondrocyte morphology, proliferation, and alignment were investigated as nanoscale CNT networks were applied to macroscopically textured polydimethylsiloxane (PDMS) scaffolds. The application of CNTs to the surface of PDMS‐based scaffolds resulted in an up to 10‐fold increase in cell adherence and 240% increase in proliferation, which is attributable to increased nanoscale roughness and hydrophilicity. The introduction of macroscale features to PDMS induced alignment of chondrocytes, successfully mimicking the cell behavior observed in the superficial layer of cartilage. Raman spectroscopy was used as a noninvasive, label‐free method to monitor extracellular matrix production and chondrocyte phenotype. Chondrocytes on these scaffolds successfully produced collagen, glycosaminoglycan, and aggrecan. This study demonstrates that introducing physical features at different length scales allows for a high level of control over tissue scaffold design and, thus, cell behavior. Ultimately, these textured scaffolds can serve as platforms to improve the understanding of osteoarthritis and for early‐stage therapeutic testing. Graphical abstract: There is a significant need to understand the complexity and heterogeneity of articular cartilage to develop more effective therapeutic strategies for diseases such as osteoarthritis. Here we show that carbon nanotubes (CNTs) are excellent candidates as a material for synthetic scaffolds to support the growth of chondrocytes. Chondrocyte morphology, proliferation, and alignment were investigated as nanoscale CNT networks were applied to macroscopically textured polydimethylsiloxane (PDMS) scaffolds.
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影响因子：
18.2
作者：
Bergholt, Mads S.;St-Pierre, Jean-Philippe;Offeddu, Giovanni S.;Parmar, Paresh A.;Albro, Michael B.;Puetzer, Jennifer L.;Oyen, Michelle L.;Stevens, Molly M.
通讯作者：
Stevens, Molly M.
影响因子：
7.2
作者：
Albro MB;Bergholt MS;St-Pierre JP;Vinals Guitart A;Zlotnick HM;Evita EG;Stevens MM
通讯作者：
Stevens MM
影响因子：
7.4
作者：
FRANK, CJ;MCCREERY, RL;REDD, DCB
通讯作者：
REDD, DCB
影响因子：
8
作者：
Das, Kinsuk;Madhusoodan, A. P.;Bag, Sadhan
通讯作者：
Bag, Sadhan
影响因子：
4.1
作者：
Chahine, Nadeen O.;Collette, Nicole M.;Loots, Gabriela G.
通讯作者：
Loots, Gabriela G.