Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage.
Raman Spectroscopy Reveals New Insights into the Zonal Organization of Native and Tissue-Engineered Articular Cartilage.
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DOI：
10.1021/acscentsci.6b00222
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发表时间：
2016-12-28
影响因子：
18.2
通讯作者：
Stevens, Molly M.
中科院分区：
文献类型：
--
作者：
Bergholt, Mads S.;St-Pierre, Jean-Philippe;Offeddu, Giovanni S.;Parmar, Paresh A.;Albro, Michael B.;Puetzer, Jennifer L.;Oyen, Michelle L.;Stevens, Molly M.
组织结构与其功能密切相关，组织组织的丧失通常与病理学有关。关节软骨中细胞外基质（ECM）复杂的深度依赖性排列对其生物力学功能至关重要。在这项研究中，我们开发了一种拉曼光谱成像方法，以获得新的洞察深度依赖性安排的天然和组织工程化的关节软骨，使用牛组织和细胞。我们的研究结果揭示了以前未报告的组织复杂性到至少六个区以上的潮标的基础上的主成分分析和k-均值聚类分析的主要ECM成分的分布和方向。纳米压痕和拉曼光谱数据的相关性表明，整个组织深度的生物力学的ECM的微观结构，而不是组成的影响。此外，拉曼光谱与多变量分析一起揭示了组织工程构建体中胶原、糖胺聚糖和水分布随时间的变化。使用简单的指标评估这些变化，这些指标有望指导具有天然区域复杂性和功能性能的广泛组织的再生。拉曼成像用于绘制天然和工程化关节软骨的生化组成和胶原蛋白取向。这种方法提供了对组织复杂性的深入了解，并可用于评估工程结构的质量。
Tissue architecture is intimately linked with its functions, and loss of tissue organization is often associated with pathologies. The intricate depth-dependent extracellular matrix (ECM) arrangement in articular cartilage is critical to its biomechanical functions. In this study, we developed a Raman spectroscopic imaging approach to gain new insight into the depth-dependent arrangement of native and tissue-engineered articular cartilage using bovine tissues and cells. Our results revealed previously unreported tissue complexity into at least six zones above the tidemark based on a principal component analysis and k-means clustering analysis of the distribution and orientation of the main ECM components. Correlation of nanoindentation and Raman spectroscopic data suggested that the biomechanics across the tissue depth are influenced by ECM microstructure rather than composition. Further, Raman spectroscopy together with multivariate analysis revealed changes in the collagen, glycosaminoglycan, and water distributions in tissue-engineered constructs over time. These changes were assessed using simple metrics that promise to instruct efforts toward the regeneration of a broad range of tissues with native zonal complexity and functional performance. Raman imaging was used to map biochemical composition and collagen orientation of native and engineered articular cartilage. This approach provides insight into tissue complexity and can be harnessed to evaluate the quality of engineered constructs.
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DOI：
10.3109/10731199509117971
发表时间：
1995-01-01
影响因子：
--
作者：
KANDEL, RA;CHEN, H;RENLUND, R
通讯作者：
RENLUND, R
影响因子：
2.5
作者：
Gamsjaeger, Sonja;Klaushofer, Klaus;Paschalis, Eleftherios P.
通讯作者：
Paschalis, Eleftherios P.
DOI：
10.1302/0301-620x.33b2.251
发表时间：
1951-01-01
影响因子：
--
作者：
MACCONAILL, MA
通讯作者：
MACCONAILL, MA
影响因子：
3.9
作者：
Kunstar, Aliz;Leferink, Anne M.;van Apeldoorn, Aart A.
通讯作者：
van Apeldoorn, Aart A.
影响因子：
2.4
作者：
Klein, Travis J.;Chaudhry, Manu;Sah, Robert L.
通讯作者：
Sah, Robert L.