OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice.
OsCESA9 conserved-site mutation leads to largely enhanced plant lodging resistance and biomass enzymatic saccharification by reducing cellulose DP and crystallinity in rice.
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DOI：
10.1111/pbi.12700
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发表时间：
2017-09
影响因子：
13.8
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Li F;Xie G;Huang J;Zhang R;Li Y;Zhang M;Wang Y;Li A;Li X;Xia T;Qu C;Hu F;Ragauskas AJ;Peng L
植物细胞壁的遗传修饰已经被提出来降低木质纤维素糖化率以增强生物质糖化。自纤维素合成酶（CESA）基因首次被发现以来，已报道了几十种CESA突变体，但几乎所有的突变体都表现出对植物生长发育不利的表型。在本研究中，在CESA 9的P-CR保守位点发生取代（W 481 C、P482 S）的水稻（Oryza sativa）Osfc 16突变体显示，与野生型（日本晴，一种粳稻品种）相比，植物生长受到轻微影响，生物量产量提高了25%-41%。化学和超微结构分析表明，Osfc 16具有显着降低纤维素结晶度（CrI）和较薄的次生细胞壁相比，野生型。CESA co-IP检测，以及蛋白酶体抑制剂（MG 132）和两种不同的纤维素抑制剂（Calcofluor，CGA）的实施，表明CESA 9突变可能影响CESA 4/7/9复合物的完整性，这可能导致CESA蛋白酶体快速降解，用于低DP纤维素生物合成。这些可以降低纤维素CrI，从而提高植物抗倒伏性，这是植物生长和谷物生产的主要综合农艺性状，并将生物质酶促糖化提高2.3倍，乙醇生产率提高34%-42%。该研究首次报道了用于低DP纤维素生产的直接改性，该改性在生物质工业中具有广泛的应用。
Genetic modification of plant cell walls has been posed to reduce lignocellulose recalcitrance for enhancing biomass saccharification. Since cellulose synthase (CESA) gene was first identified, several dozen CESA mutants have been reported, but almost all mutants exhibit the defective phenotypes in plant growth and development. In this study, the rice (Oryza sativa) Osfc16 mutant with substitutions (W481C, P482S) at P‐CR conserved site in CESA9 shows a slightly affected plant growth and higher biomass yield by 25%–41% compared with wild type (Nipponbare, a japonica variety). Chemical and ultrastructural analyses indicate that Osfc16 has a significantly reduced cellulose crystallinity (CrI) and thinner secondary cell walls compared with wild type. CESA co‐IP detection, together with implementations of a proteasome inhibitor (MG132) and two distinct cellulose inhibitors (Calcofluor, CGA), shows that CESA9 mutation could affect integrity of CESA4/7/9 complexes, which may lead to rapid CESA proteasome degradation for low‐DP cellulose biosynthesis. These may reduce cellulose CrI, which improves plant lodging resistance, a major and integrated agronomic trait on plant growth and grain production, and enhances biomass enzymatic saccharification by up to 2.3‐fold and ethanol productivity by 34%–42%. This study has for the first time reported a direct modification for the low‐DP cellulose production that has broad applications in biomass industries.
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影响因子：
56.9
作者：
Peng, LC;Kawagoe, Y;Delmer, D
通讯作者：
Delmer, D
DOI：
10.1073/pnas.1202079109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Chiniquy, Dawn;Sharma, Vaishali;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.
影响因子：
11.4
作者：
Li, Ming;Si, Shengli;Peng, Liangcai
通讯作者：
Peng, Liangcai
DOI：
10.1073/pnas.1200352109
发表时间：
2012-03-13
影响因子：
11.1
作者：
Harris, Darby M.;Corbin, Kendall;DeBolt, Seth
通讯作者：
DeBolt, Seth
影响因子：
2
作者：
CROOK, MJ;ENNOS, AR
通讯作者：
ENNOS, AR