In vitro elution characteristics of gentamicin-impregnated Polymethylmethacrylate: premixed with a second powder vs. liquid Lyophilization.
In vitro elution characteristics of gentamicin-impregnated Polymethylmethacrylate: premixed with a second powder vs. liquid Lyophilization.
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DOI：
10.1186/s12891-020-03923-w
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发表时间：
2021-01-04
影响因子：
2.3
通讯作者：
Yuenyongviwat V
中科院分区：
文献类型：
--
作者：
Liawrungrueang W;Ungphaiboon S;Jitsurong A;Ingviya N;Tangtrakulwanich B;Yuenyongviwat V
抗生素负载骨水泥或抗生素浸渍的聚甲基丙烯酸甲酯（PMMA）被开发用于预防和治疗骨和关节感染。庆大霉素是一种抗生素，通常与PMMA联合使用；然而，庆大霉素粉末在许多国家很难买到。本研究旨在评价庆大霉素冻干液制备庆大霉素浸渍PMMA的洗脱特性，并与PMMA进行比较；它是由商业庆大霉素粉末制成的。实验样品分为庆大霉素粉剂组（PG-PMMA）和冻干庆大霉素液体组（LG-PMMA） 2组。每组配制10个水泥隔离剂。在加入液体单体（2g庆大霉素和40g PMMA）之前，将庆大霉素粉末或冻干庆大霉素液体与粉末聚合物混合。抗生素浸渍水泥垫片的体积和表面积分别为50 cm3和110 cm2。每个隔离液浸泡在磷酸盐缓冲盐水中，在无菌条件下每天更换一次。收集溶液，在第1、2、3、4、5、6、7、14、21、28、35和42天采用酶倍增免疫分析法（EMIT）检测庆大霉素水平。两组标本第1天庆大霉素浓度均较高（LG-PMMA为113.63±23.42 mg/dL， PG-PMMA为61.7±8.37 mg/dL），但随着时间的推移持续下降。两组PMMA间隔剂释放庆大霉素的时间最长可达6周（PG-PMMA为3.28±1.17 mg/dL， PG-PMMA为1.21±0.28 mg/dL）。在各时间点，LG-PMMA组庆大霉素浓度均显著高于PG-PMMA组（P< 0.05）。在初步的体外研究中，用冻干庆大霉素液体制成的庆大霉素浸渍PMMA的抗生素洗脱率大约是用预混庆大霉素粉末制成的PMMA的两倍。
Antibiotic-loaded bone cement, or antibiotic-impregnated polymethylmethacrylate (PMMA), were developed to prevent and treat bone and joint infections. Gentamicin is an antibiotic that is commonly used in combination with PMMA; however, gentamicin powder is hard to obtain in many countries. This study aimed to evaluate the elution characteristics of gentamicin-impregnated PMMA made with lyophilized liquid gentamicin, compared with PMMA; which is made from commercial gentamicin powder. The experimental sample was divided into 2 groups: the gentamicin power group (PG-PMMA) and the lyophilized liquid gentamicin group (LG-PMMA). Ten cement spacers were prepared in each group. These were produced by mixing gentamicin powder, or lyophilized liquid gentamicin, with a powder polymer before adding the liquid monomer (2 g of gentamicin and 40 g of PMMA). The volume and surface area of the antibiotic-impregnated cement spacers were 50 cm3 and 110 cm2, respectively. Each spacer was immersed in phosphate-buffered saline, which was changed daily under sterile conditions. The solutions were collected to measure the level of gentamicin using the enzyme multiplied immunoassay technique (EMIT), at days 1, 2, 3, 4, 5, 6, 7, 14, 21, 28, 35 and 42. The collections from both groups had high concentrations of gentamicin on day 1 (113.63 ± 23.42 mg/dL in LG-PMMA and 61.7 ±8.37 mg/dL in PG-PMMA), but experienced a continuous decrease over time. The PMMA spacers from both groups could release gentamicin for up to 6 weeks (3.28 ± 1.17 mg/dL in LG-PMMA and 1.21 ± 0.28 mg/dL in PG-PMMA). However, there were significantly higher levels of gentamicin concentrations in the LG-PMMA group compared to the PG-PMMA group at all time points (P< 0.05). Gentamicin-impregnated PMMA made with lyophilized liquid gentamicin had approximately a two times higher rate of antibiotic elution in preliminary in vitro studies, as compared with PMMA made with premixed gentamicin powder.
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DOI：
10.5402/2011/290851
发表时间：
2011
期刊：
ISRN orthopedics
影响因子：
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