Evidence for catecholaminergic, neuronlike cells in the adult human testis: changes associated with testicular pathologies.
Evidence for catecholaminergic, neuronlike cells in the adult human testis: changes associated with testicular pathologies.
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成人睾丸中儿茶酚胺能神经元样细胞的证据：与睾丸病理相关的变化。
DOI：
10.1002/j.1939-4640.1999.tb02527.x
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发表时间：
1999
影响因子：
--
通讯作者：
Vogt Hj
中科院分区：
文献类型：
--
作者：
Artur Mayerhofer;M. Frungieri;S. Fritz;Andreas Bulling;B. Jessberger;Vogt Hj
最近在非人类灵长类动物——恒河猴的睾丸中发现了表达酪氨酸羟化酶（TH）的神经元样儿茶酚胺能细胞。我们检查了人类睾丸中是否存在神经元样细胞。为此，我们首先确定 TH 和电压激活钠通道 (NaCh) 的基因（神经元兴奋性的先决条件）是否在正常成人睾丸中表达。使用 RT-PCR 方法，分离出与人 TH 序列和 NaCh 型 α 亚基相同的 cDNA 克隆。免疫组织化学方法在无睾丸病变的成年男性（年龄范围，28-44 岁）和患有仅支持细胞 (SCO) 综合征或严重精子生成不足和生殖细胞停滞 (GA) 的不育患者的睾丸活检中定位了相应的蛋白质。 TH 和 NaCh 抗体以及识别多巴胺转运蛋白的抗体在每组的大多数（但不是全部）活组织检查中（23 个中的 12 个）鉴定出主要为双极或偶尔为多极、拉长表型的免疫反应细胞。通过 RT-PCR 方法对活检组织中 TH 基因表达的鉴定证实了结果。在 SCO 和 GA 活检中更容易检测到免疫反应性细胞体以及神经纤维。在从所有活检中随机选择的一组切片中使用神经丝 200 (NF-200) 单克隆抗体对所有睾丸神经元元件、细胞体和纤维进行免疫组织化学可视化后进行量化。我们发现 SCO 综合征和 GA 活检中 NF-200 免疫反应性细胞体和纤维显着增加。这些结果表明，人类睾丸中存在一种尚不清楚的睾丸儿茶酚胺能神经元样细胞类型。这种细胞类型可以补充众所周知的睾丸交感神经支配并与其协同作用。病理睾丸活检中“内在”（神经元细胞）和“外在”（神经纤维）神经元成分的增加表明，人类睾丸神经系统的两个部分可能参与 GA 和 SCO 综合征的发病机制和/或维持。
Neuronlike, catecholaminergic cells expressing tyrosine-hydroxylase (TH) have recently been found in the testis of a nonhuman primate species, the rhesus monkey. We examined whether neuronlike cells are present in the human testis. To this end, we first determined if the genes for TH and for a voltage-activated sodium channel (NaCh), a prerequisite for neuronal excitability, are expressed in normal adult testes. Using an RT-PCR approach, cDNA clones, identical to the sequences of human TH and to the alpha subunit of a NaCh type, were isolated. Immunohistochemical methods localized the corresponding proteins in testicular biopsies from adult men (age range, 28-44 years) without testicular pathologies and from infertile patients with either Sertoli cell only (SCO) syndrome or severe hypospermatogenesis and germ cell arrest (GA). TH and NaCh antibodies, as well as antibodies recognizing dopamine-transporter protein, identified immunoreactive cells of mainly bipolar or occasionally multipolar, elongated phenotype in most, but not all, biopsies of each group (12 out of 23). The results were corroborated by identification of TH gene expression by RT-PCR approaches in biopsies. Immunoreactive cell bodies, as well as nerve fibers, were more readily detected in SCO and GA biopsies. This was quantified after immunohistochemically visualizing all testicular neuronal elements, cell bodies, and fibers, with a neurofilament 200 (NF-200) monoclonal antibody in one set of randomly selected sections from all biopsies. We found significantly increased NF-200-immunoreactive cell bodies and fibers in SCO-syndrome and GA biopsies. These results show the existence of an as yet unknown testicular catecholaminergic neuronlike cell type in the human testis. This cell type may complement and act in concert with the well-known testicular sympathetic innervation. The increase of both "intrinsic" (neuronal cells) and "extrinsic" (nerve fibers) neuronal elements in pathological testicular biopsies suggests that the two parts of the human testicular nervous system may be involved in pathogenesis and/or maintenance of GA and SCO syndromes.
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大鼠睾丸的传出神经支配。
影响因子：
3.6
作者：
Rauchenwald,M;Steers,WD;Desjardins,C
通讯作者：
Desjardins,C
影响因子：
3.6
作者：
MAYERHOFER, A;BARTKE, A;STEGER, RW
通讯作者：
STEGER, RW
DOI：
10.1073/pnas.94.4.1527
发表时间：
1997-02-18
影响因子：
11.1
作者：
ToledoAral, JJ;Moss, BL;Mandel, G
通讯作者：
Mandel, G
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影响因子：
3.6
作者：
Mayerhofer,A;Bartke,A;Began,T
通讯作者：
Began,T