Stem cell- and scaffold-based tissue engineering approaches to osteochondral regenerative medicine.
Stem cell- and scaffold-based tissue engineering approaches to osteochondral regenerative medicine.
复制标题
基于干细胞和脚手架的组织工程方法，用于骨软骨再生医学。
DOI：
10.1016/j.semcdb.2009.03.017
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发表时间：
2009-08
影响因子：
7.3
通讯作者：
Kaplan DL
中科院分区：
文献类型：
--
作者：
Sundelacruz S;Kaplan DL
在骨软骨组织工程中，细胞的募集、增殖、分化和图案化是形成生物和结构上可行的结构以修复受损或患病组织的关键。然而，由于体外制备的结构缺乏体内微环境中存在的大量线索，细胞通常需要外部生物和物理刺激来诱导它们实现目标组织功能。为了确定将哪些刺激呈现给细胞，生物工程策略可以从内源性骨骼形成的例子中受益匪浅。作为发育成骨的一个例子，发育中的肢芽是研究骨软骨结构如何由未分化的前体细胞形成的一个很好的模型系统。除了胚胎发育过程中的骨骼形成，骨骼还具有先天的再生能力，表现出显著的损伤后愈合能力。骨折愈合与骨发育有许多共同的特征，这推动了再生过程通常是对发育的概括的假说。这两种骨形成模式的异同可能有助于深入了解修复受损或患病的骨的特殊要求。因此，内源性骨折愈合作为再生骨骼形成的一个例子，也可能为生物工程策略提供参考。在这篇综述中，我们总结了干细胞和祖细胞在发育和再生骨骼形成中涉及的关键细胞事件，并平行讨论了组织工程师在体外总结这些事件所采用的相应的基于细胞和支架的策略。
In osteochondral tissue engineering, cell recruitment, proliferation, differentiation, and patterning are critical for forming biologically and structurally viable constructs for repair of damaged or diseased tissue. However, since constructs prepared ex vivo lack the multitude of cues present in the in vivo microenvironment, cells often need to be supplied with external biological and physical stimuli to coax them towards targeted tissue functions. To determine which stimuli to present to cells, bioengineering strategies can benefit significantly from endogenous examples of skeletogenesis. As an example of developmental skeletogenesis, the developing limb bud serves as an excellent model system in which to study how an osteochondral structures form from undifferentiated precursor cells. Alongside skeletal formation during embryogenesis, bone also possesses innate regenerative capacity, displaying remarkable ability to heal after damage. Bone fracture healing shares many features with bone development, driving the hypothesis that the regenerative process generally recapitulates development. Similarities and differences between the two modes of bone formation may offer insight into the special requirements for healing damaged or diseased bone. Thus, endogenous fracture healing, as an example of regenerative skeletogenesis, may also inform bioengineering strategies. In this review, we summarize the key cellular events involving stem and progenitor cells in developmental and regenerative skeletogenesis, and discuss in parallel the corresponding cell- and scaffold-based strategies that tissue engineers employ to recapitulate these events in vitro.
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