Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling.
Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling.
复制标题
DOI：
10.1186/s12864-015-1405-8
复制
发表时间：
2015-03-19
期刊：
影响因子：
4.4
通讯作者：
Hansen JB
中科院分区：
文献类型：
--
作者：
Basse AL;Dixen K;Yadav R;Tygesen MP;Qvortrup K;Kristiansen K;Quistorff B;Gupta R;Wang J;Hansen JB
由于棕色脂肪组织（BAT）的存在，大型哺乳动物在出生后不久就能够进行体温调节。大部分BAT在出生后消失，并被白色脂肪组织（WAT）所取代。我们分析了绵羊出生后的脂肪转化与肾周脂肪库的时间过程研究。我们观察到组织形态，基因表达和代谢的变化在出生后的前两周内与预期的从BAT到WAT的过渡一致。这种转化的特征在于线粒体丰度的大量减少以及与线粒体功能和氧化磷酸化相关的基因表达的下调。总体基因表达谱显示，时间点分为三个阶段：棕色脂肪阶段、过渡阶段和白色脂肪阶段。在棕色脂肪和过渡期之间有170个基因差异表达，在过渡期和白色脂肪期之间有717个基因差异表达。两组差异表达基因共有38个基因。我们鉴定了许多受调控的转录因子，包括NR 1H 3、MYC、KLF 4、ESR 1、RELA和BCL 6，它们与脂肪组织重塑期间基因表达的总体变化有关。最后，肾周脂肪组织在出生时表达棕色和浅褐色/米色脂肪细胞标记基因，其表达随时间推移而发生实质性变化。使用全球基因表达谱的出生后BAT到WAT的转换在羊，我们提供了新的见解脂肪组织可塑性在大型哺乳动物，包括识别新的转录成分与脂肪组织重塑。此外，我们的数据集提供了一个有用的资源，在脂肪组织可塑性的进一步研究。本文的在线版本（doi：10.1186/s12864-015-1405-8）包含补充材料，可供授权用户使用。
Large mammals are capable of thermoregulation shortly after birth due to the presence of brown adipose tissue (BAT). The majority of BAT disappears after birth and is replaced by white adipose tissue (WAT). We analyzed the postnatal transformation of adipose in sheep with a time course study of the perirenal adipose depot. We observed changes in tissue morphology, gene expression and metabolism within the first two weeks of postnatal life consistent with the expected transition from BAT to WAT. The transformation was characterized by massively decreased mitochondrial abundance and down-regulation of gene expression related to mitochondrial function and oxidative phosphorylation. Global gene expression profiling demonstrated that the time points grouped into three phases: a brown adipose phase, a transition phase and a white adipose phase. Between the brown adipose and the transition phase 170 genes were differentially expressed, and 717 genes were differentially expressed between the transition and the white adipose phase. Thirty-eight genes were shared among the two sets of differentially expressed genes. We identified a number of regulated transcription factors, including NR1H3, MYC, KLF4, ESR1, RELA and BCL6, which were linked to the overall changes in gene expression during the adipose tissue remodeling. Finally, the perirenal adipose tissue expressed both brown and brite/beige adipocyte marker genes at birth, the expression of which changed substantially over time. Using global gene expression profiling of the postnatal BAT to WAT transformation in sheep, we provide novel insight into adipose tissue plasticity in a large mammal, including identification of novel transcriptional components linked to adipose tissue remodeling. Moreover, our data set provides a useful resource for further studies in adipose tissue plasticity. The online version of this article (doi:10.1186/s12864-015-1405-8) contains supplementary material, which is available to authorized users.
登录
查看更多内容
DOI：
10.1071/bi9780505
发表时间：
1978-01-01
期刊：
AUSTRALIAN JOURNAL OF BIOLOGICAL SCIENCES
影响因子：
--
作者：
GEMMELL, RT;ALEXANDER, G
通讯作者：
ALEXANDER, G
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
14.9
作者：
Flicek P;Ahmed I;Amode MR;Barrell D;Beal K;Brent S;Carvalho-Silva D;Clapham P;Coates G;Fairley S;Fitzgerald S;Gil L;García-Girón C;Gordon L;Hourlier T;Hunt S;Juettemann T;Kähäri AK;Keenan S;Komorowska M;Kulesha E;Longden I;Maurel T;McLaren WM;Muffato M;Nag R;Overduin B;Pignatelli M;Pritchard B;Pritchard E;Riat HS;Ritchie GR;Ruffier M;Schuster M;Sheppard D;Sobral D;Taylor K;Thormann A;Trevanion S;White S;Wilder SP;Aken BL;Birney E;Cunningham F;Dunham I;Harrow J;Herrero J;Hubbard TJ;Johnson N;Kinsella R;Parker A;Spudich G;Yates A;Zadissa A;Searle SM
通讯作者：
Searle SM
影响因子：
18.2
作者：
Kajimura S;Saito M
通讯作者：
Saito M
影响因子：
16
作者：
Campbell, KJ;Rocha, S;Perkins, ND
通讯作者：
Perkins, ND