Quantum dots trigger immunomodulation of the NFκB pathway in human skin cells.
Quantum dots trigger immunomodulation of the NFκB pathway in human skin cells.
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DOI：
10.1016/j.molimm.2011.02.009
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发表时间：
2011-07
影响因子：
3.6
通讯作者：
Sayes CM
中科院分区：
文献类型：
--
作者：
Romoser AA;Chen PL;Berg JM;Seabury C;Ivanov I;Criscitiello MF;Sayes CM
量子点的免疫效应取决于多种因素，包括但不限于暴露时间和给药浓度。在这项研究中，我们研究了15 nm的CdSe/ZnS-COOH量子点纳米晶体（QDs）对人表皮角质形成细胞（HEK）和人真皮成纤维细胞（HDF）的细胞密度，活力和形态的影响。此外，使用蛋白质和真实的时间PCR阵列分析，从暴露于预定亚致死浓度QD的HDF细胞测量炎症和非炎症免疫应答。CdSe/ZnS-COOH量子点引起浓度依赖性（1-120 nM暴露浓度）和时间依赖性（8小时或48小时）的细胞死亡，证明了代谢活性和形态学变化。QD暴露诱导凋亡、炎症和免疫调节蛋白（如TNF-α、IL-1B和IL-10）上调。HMOX 1是活性氧中间体（ROI）和/或金属引起的应激指标，在随后的时间点也上调。QD还引起已知与炎症（IL 1-β、CCL 2、IRAK-2）、免疫（IL-1、IL-6、PGLYRP 1、SERPINA 1、IL-10）、ROI和/或重金属（HMOX 1）所致应激和凋亡（CASP 1、ADORA 2A）反应相关的基因的调节。发现QD暴露的细胞效应主要遵循NFκB途径。此外，QD在不同的暴露浓度和时间点诱导角质形成细胞和成纤维细胞的差异细胞毒性，即使在生理相关的给药浓度，因此强调需要研究在同一靶器官内不同细胞类型之间的潜在作用机制。
The immunological effects of quantum dots are dependent on a variety of factors including, but not limited to, exposure time and dosing concentrations. In this study, we investigated the influence of 15 nm CdSe/ZnS-COOH quantum dot nanocrystals (QDs) on cell density, viability, and morphology in human epidermal keratinocytes (HEK) and human dermal fibroblasts (HDF). Furthermore, inflammatory and non-inflammatory immune responses were measured using protein and real time PCR array analysis from HDF cells exposed to predetermined sub-lethal concentrations of QDs. CdSe/ZnS-COOH QDs caused concentration-dependent (1–120 nM exposure concentrations) and time-dependent (8 h or 48 h) cell death, as evidenced by metabolic activity and morphological changes. QD exposure induced upregulation of apoptotic, inflammatory and immunoregulatory proteins such as TNF-α, IL-1B and IL-10. HMOX1, an indicator of stress due to reactive oxygen intermediates (ROIs) and/or metals, was upregulated at the later time point as well. QDs also caused modulation of genes known to be associated with inflammatory (IL1-β,CCL2,IRAK-2), immune (IL-1,IL-6,PGLYRP1,SERPINA1,IL-10), stress due to ROIs and/or heavy metals (HMOX1), and apoptotic (CASP1,ADORA2A) responses. Cellular effects from QD exposure were found to primarily follow the NFκB pathway. In addition, QDs induced a differential cytotoxicity in keratinocytes and fibroblasts at different exposure concentrations and time points, even at physiologically relevant dosing concentrations, thus emphasizing the need to investigate potential mechanisms of action among different cell types within the same target organ.
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影响因子：
4.3
作者：
Graham, B;Gibson, SB
通讯作者：
Gibson, SB
DOI：
10.1006/bbrc.2000.2287
发表时间：
2000-03-16
影响因子：
3.1
作者：
Huber, MA;Kraut, N;Peter, RU
通讯作者：
Peter, RU
影响因子：
15
作者：
Duan, Hongwei;Nie, Shuming
通讯作者：
Nie, Shuming
影响因子：
46.9
作者：
Jaiswal, JK;Mattoussi, H;Simon, SM
通讯作者：
Simon, SM
影响因子：
4.7
作者：
Delehanty, James B.;Medintz, Igor L.;Mattoussi, Hedi
通讯作者：
Mattoussi, Hedi