Neonatal SVZ EGFP-labeled cells produce neurons in the olfactory bulb and astrocytes in the cerebral cortex by in-vivo electroporation
Neonatal SVZ EGFP-labeled cells produce neurons in the olfactory bulb and astrocytes in the cerebral cortex by in-vivo electroporation
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新生儿 SVZ EGFP 标记细胞通过体内电穿孔产生嗅球神经元和大脑皮层星形胶质细胞
DOI：
10.1097/wnr.0b013e328360f7ef
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发表时间：
2013
期刊：
影响因子：
1.7
通讯作者：
Jing Wang
中科院分区：
文献类型：
--
作者：
Xi;Li;Zhibao Guo;Wenbin Li;Wei Liu;Bao;Jing Wang
神经祖细胞/干细胞（Neural progenitor/stem cells，NSCs）存在于新生小鼠脑室下区（subventricular zone，SVZ）。为探讨新生小鼠侧脑室（SVZ）神经干细胞的分化及其子代细胞在成年早期的分布，采用体内电穿孔法将增强型绿色荧光蛋白（EGFP）质粒导入新生小鼠侧脑室（P0）神经干细胞中，并追踪其子代细胞。电穿孔后30天，制备小鼠脑的组织切片，用细胞特异性抗体进行免疫荧光，以鉴定EGFP标记的细胞类型。结果显示，EGFP阳性细胞主要分布于嗅球、皮层和SVZ，嗅球中的EGFP阳性细胞与NeuN（神经元标记物）、皮层中的胶质细胞酸性蛋白（GFAP）（星形胶质细胞标记物）、SVZ中的Blbp和GFAP（星形胶质细胞标记物）呈双标记。海马区未见EGFP阳性细胞。本研究结果表明，新生小鼠SVZ中的神经干细胞在成年早期可以产生OB中的神经元和皮质中的星形胶质细胞，但不能产生驻留在海马中的子代细胞。此外，直到成年早期，SVZ中仍然存在神经祖细胞。
Neural progenitors/stem cells (NSCs) exist in neonatal mouse subventricular zone (SVZ). To explore the differentiation of the NSCs in neonatal mouse SVZ and the distribution of the progeny cells derived from these NSCs in early adulthood, the enhanced green fluorescent protein (EGFP) plasmid was transferred into the NSCs in the lateral ventricle of newborn mice (P0) by in-vivo electroporation to trace these cells and their progeny cells. Thirty days after electroporation, histological sections of mouse brain were prepared for immunofluorescence with cell-specific antibodies to identify the type(s) of cells that were marked by EGFP. The results showed that EGFP-positive cells were distributed mainly in the olfactory bulb (OB), cortex, and SVZ, and double labeled with NeuN (neuron marker) in OB, glial fibrillary acidic protein (GFAP) (astrocyte marker) in the cortex, and Blbp and GFAP (astrocyte marker) in SVZ. However, there was no-EGFP-positive cell in the hippocampus. The present results indicate that the NSCs in SVZ of the neonatal mouse can give rise to neurons in the OB and astrocytes in the cortex in early adulthood, but not generate progeny cells residing in the hippocampus. In addition, there are still neural progenitors in SVZ until early adulthood.
DOI：
10.1073/pnas.250471697
发表时间：
2000-12-05
影响因子：
11.1
作者：
Laywell, ED;Rakic, P;Steindler, DA
通讯作者：
Steindler, DA
新生儿室下区的神经胶质祖细胞异步分化，导致神经胶质克隆的空间分散以及成年哺乳动物中枢神经系统中未成熟神经胶质的持续存在。
DOI：
10.1016/j.ydbio.2004.02.024
发表时间：
2004
期刊：
Developmental biology.
影响因子：
--
作者：
Zerlin,Marielba;Milosevic,Ana;Goldman,JamesE
通讯作者：
Goldman,JamesE
影响因子：
4.6
作者：
Xin, HB;Deng, KY;Kotlikoff, MI
通讯作者：
Kotlikoff, MI